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Synthesis of gemifloxacin conjugated silver nanoparticles, their amplified bacterial efficacy against human pathogen and their morphological study via TEM analysis.
Ahmad, Touqeer; Mahbood, Fazal; Sarwar, Rizwana; Iqbal, Ayesha; Khan, Majid; Muhammad, Sayyar; Al-Riyami, Khamis; Hussain, Nusrat; Uddin, Jalal; Khan, Ajmal; Al-Harrasi, Ahmed.
Afiliación
  • Ahmad T; Natural and Medical Sciences Research Center, University of Nizwa, Nizwa, Oman.
  • Mahbood F; Institute of Chemical Sciences, University of Swat, KP, Pakistan.
  • Sarwar R; Department of Chemistry, COMSATS University Islamabad Abbottabad Campus, Abbottabad, Pakistan.
  • Iqbal A; Division of Pharmacy Practice and Policy, School of Pharmacy, University of Nottingham, Nottingham, UK.
  • Khan M; Natural and Medical Sciences Research Center, University of Nizwa, Nizwa, Oman.
  • Muhammad S; International Center for Chemical and Biological Sciences, H.E.J. Research Institute of Chemistry, University of Karachi, Karachi, Pakistan.
  • Al-Riyami K; Department of Chemistry, Islamia College, Peshawar, Pakistan.
  • Hussain N; Natural and Medical Sciences Research Center, University of Nizwa, Nizwa, Oman.
  • Uddin J; Department of Chemistry, University of Baltistan Skardu, Skardu, Pakistan.
  • Khan A; Department of Pharmaceutical Chemistry, College of Pharmacy, King Khalid University, Abha, Saudi Arabia.
  • Al-Harrasi A; Natural and Medical Sciences Research Center, University of Nizwa, Nizwa, Oman.
Artif Cells Nanomed Biotechnol ; 49(1): 661-671, 2021 Dec.
Article en En | MEDLINE | ID: mdl-34818127
Drug-loaded nanoparticles (NPs) allow specific accumulation and controlled release of drugs to infected tissues with minimal cytotoxicity. In this study, gemifloxacin conjugated silver nanoparticles (Gemi-AgNPs) were synthesized, and the amplification of their antibacterial potential against the human pathogen as well as their stability was monitored under physiological conditions. Fourier transform infrared spectroscopy (FTIR) analysis demonstrated the interaction between -NH2 and -OH functional moiety and the metal surface. The morphological analyses via transmission electron microscopy revealed that Gemi-AgNPs has a round oval shape and average particle size of 22.23 ± 2 nm. The antibacterial and antibiofilm activities of these NPS showed that Gemi-AgNPs exhibit excellent antimicrobial and biofilm inhibition activity against human pathogens, namely, Proteus mirabilis (P. mirabilis) and methicillin-resistant Staphylococcus aureus (MRSA). A significant increase in the antibiofilm activity of Gemi-AgNPs was confirmed by crystal violet, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) staining, and microscopic analysis. Gemi-AgNPs exhibited the ability to inhibit urease with an IC50 value of 57.4 ± 0.72 µg/mL. The changes in the bacterial cell morphology were analyzed via TEM, which revealed that cell membranes disrupted and completely destroyed the cell morphology by the treatment of Gemi-AgNPs.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nanopartículas del Metal / Staphylococcus aureus Resistente a Meticilina Límite: Humans Idioma: En Revista: Artif Cells Nanomed Biotechnol Año: 2021 Tipo del documento: Article País de afiliación: Omán Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nanopartículas del Metal / Staphylococcus aureus Resistente a Meticilina Límite: Humans Idioma: En Revista: Artif Cells Nanomed Biotechnol Año: 2021 Tipo del documento: Article País de afiliación: Omán Pais de publicación: Reino Unido