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Single-step purified R-phycoerythrin transmits cellular imaging functionalities in vitro.
Sathuvan, Malairaj; Thangam, Ramar; Venkateshbabu, Gopal; Cheong, Kit-Leong; Kang, Heemin; Liu, Yang.
Afiliación
  • Sathuvan M; Department of Biology & Guangdong Provincial Key Laboratory of Marine Biotechnology, Institute of Marine Sciences, College of Science, Shantou University, Shantou, Guangdong 515063, PR China.
  • Thangam R; Department of Materials Science and Engineering, Korea University, Seoul, 02841, Republic of Korea.
  • Venkateshbabu G; Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai-600 025, Tamil Nadu, India.
  • Cheong KL; Department of Biology & Guangdong Provincial Key Laboratory of Marine Biotechnology, Institute of Marine Sciences, College of Science, Shantou University, Shantou, Guangdong 515063, PR China.
  • Kang H; Department of Materials Science and Engineering, Korea University, Seoul, 02841, Republic of Korea.
  • Liu Y; Department of Biology & Guangdong Provincial Key Laboratory of Marine Biotechnology, Institute of Marine Sciences, College of Science, Shantou University, Shantou, Guangdong 515063, PR China. Electronic address: liuyanglft@stu.edu.cn.
Int J Biol Macromol ; 194: 563-570, 2022 Jan 01.
Article en En | MEDLINE | ID: mdl-34813785
A single-step and rapid chromatographic method-based purification of Gracilaria corticata (J. Agardh) R-phycoerythrin (R-PE) was attained using polyacrylamide gel electrophoresis (PAGE) technique without affecting structural integrity. The purified R-PE had a characteristic UV-Vis spectrum with three absorbance maxima at 496, 535, and 565 nm, and fluorescence at 575 nm. R-PE was obtained with a purity index of 4.2 and a recovery yield of 44.3%. SDS-PAGE analysis exhibited three sub-units i.e., 18, 21, and 31 kDa, which corresponds to α, ß, and γ, respectively. This report's purification process was considered less time-consuming and could be efficiently applied to purify phycobiliproteins. The purified R-PE showed optimal stability up to 6 h at pH 7.0 when exposed to light (3000 lx), while the temperature at which the maximum stability was retained was at 20 °C. The cellular imaging property of R-PE was effectively implemented to evaluate its credentials without affecting the cell proliferation of Vero and Hep-2 cell lines with the higher IC50 concentrations in vitro. Under fluorescence microscopy and flow cytometry analysis, purified R-PE displayed the characteristic affinity towards cell imaging functions in preliminary in vitro studies.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ficoeritrina / Gracilaria / Imagen Óptica / Colorantes Fluorescentes Límite: Animals / Humans Idioma: En Revista: Int J Biol Macromol Año: 2022 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ficoeritrina / Gracilaria / Imagen Óptica / Colorantes Fluorescentes Límite: Animals / Humans Idioma: En Revista: Int J Biol Macromol Año: 2022 Tipo del documento: Article Pais de publicación: Países Bajos