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Transplantation of human embryonic stem cells alleviates motor dysfunction in AAV2-Htt171-82Q transfected rat model of Huntington's disease.
Islam, Jaisan; So, Kyoung Ha; Kc, Elina; Moon, Hyeong Cheol; Kim, Aryun; Hyun, Sang Hwan; Kim, Soochong; Park, Young Seok.
Afiliación
  • Islam J; Department of Neuroscience, College of Medicine, Chungbuk National University, Cheongju, Republic of Korea.
  • So KH; Institute for Stem Cell & Regenerative Medicine (ISCRM), College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of Korea.
  • Kc E; Department of Neuroscience, College of Medicine, Chungbuk National University, Cheongju, Republic of Korea.
  • Moon HC; Department of Neurosurgery, Gammaknife Icon Center, Chungbuk National University Hospital, Cheongju, Republic of Korea.
  • Kim A; Department of Neurology, Chungbuk National University Hospital, Cheongju, Republic of Korea.
  • Hyun SH; Institute for Stem Cell & Regenerative Medicine (ISCRM), College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of Korea.
  • Kim S; Laboratory of Veterinary Embryology and Biotechnology (VETEMBIO), College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of Korea.
  • Park YS; Institute for Stem Cell & Regenerative Medicine (ISCRM), College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of Korea.
Stem Cell Res Ther ; 12(1): 585, 2021 11 22.
Article en En | MEDLINE | ID: mdl-34809707
BACKGROUND: Human embryonic stem cells (hESCs) transplantation had shown to provide a potential source of cells in neurodegenerative disease studies and lead to behavioral recovery in lentivirus transfected or, toxin-induced Huntington's disease (HD) rodent model. Here, we aimed to observe if transplantation of superparamagnetic iron oxide nanoparticle (SPION)-labeled hESCs could migrate in the neural degenerated area and improve motor dysfunction in an AAV2-Htt171-82Q transfected Huntington rat model. METHODS: All animals were randomly allocated into three groups at first: HD group, sham group, and control group. After six weeks, the animals of the HD group and sham group were again divided into two subgroups depending on animals receiving either ipsilateral or contralateral hESCs transplantation. We performed cylinder test and stepping test every two weeks after AAV2-Htt171-82Q injection and hESCs transplantation. Stem cell tracking was performed once per two weeks using T2 and T2*-weighted images at 4.7 Tesla MRI. We also performed immunohistochemistry and immunofluorescence staining to detect the presence of hESCs markers, huntingtin protein aggregations, and iron in the striatum. RESULTS: After hESCs transplantation, the Htt virus-injected rats exhibited significant behavioral improvement in behavioral tests. SPION labeled hESCs showed migration with hypointense signal in MRI. The cells were positive with ßIII-tubulin, GABA, and DARPP32. CONCLUSION: Collectively, our results suggested that hESCs transplantation can be a potential treatment for motor dysfunction of Huntington's disease.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedad de Huntington / Enfermedades Neurodegenerativas / Células Madre Embrionarias Humanas Límite: Animals / Humans Idioma: En Revista: Stem Cell Res Ther Año: 2021 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedad de Huntington / Enfermedades Neurodegenerativas / Células Madre Embrionarias Humanas Límite: Animals / Humans Idioma: En Revista: Stem Cell Res Ther Año: 2021 Tipo del documento: Article Pais de publicación: Reino Unido