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DGUOK-AS1 promotes cervical squamous cell carcinoma progression by suppressing miR-499a-5p that targets SPRR1B in vitro.
Song, Yan; Pan, Hongjuan; Yang, Ling; Fan, Yan; Zhang, Hui; Pan, Meichen; Zhang, Yali.
Afiliación
  • Song Y; Department of Obstetrics, Taikang Tongji (Wuhan) Hospital, Wuhan, 430050, China.
  • Pan H; Department of Obstetrics, Taikang Tongji (Wuhan) Hospital, Wuhan, 430050, China.
  • Yang L; Department of Gynaecology, Shenzhen Baoan Shiyan People's Hospital, Shenzhen, 518108, China.
  • Fan Y; Department of Obstetrics, Taikang Tongji (Wuhan) Hospital, Wuhan, 430050, China.
  • Zhang H; Department of Obstetrics, Taikang Tongji (Wuhan) Hospital, Wuhan, 430050, China.
  • Pan M; Department of Obstetrics, Taikang Tongji (Wuhan) Hospital, Wuhan, 430050, China.
  • Zhang Y; Department of Gynaecology, Shenzhen Longhua Maternal and Child Health Hospital, Shenzhen, 518110, China. Electronic address: yalizhang28@163.com.
Biochem Biophys Res Commun ; 585: 177-184, 2021 12 31.
Article en En | MEDLINE | ID: mdl-34808501
PURPOSE: Cervical squamous cell carcinoma (CESC) is the most common cancer type of cervical cancer, which threatens women's life seriously. LncRNA DGUOK-AS1has been reported to promote the biologic processes of CESC. We aim to figure out the role of DGUOK-AS1-miR-499a-5p-SPRR1B axis in modulating the CESC progression in vitro. METHODS: The levels of DGUOK-AS1, miR-499a-5p, and SPRR1B in CESC tissues and cells were examined by RT-qPCR. The interaction of DGUOK-AS1-miR-499a-5p-SPRR1B was verified by luciferase assay. Inhibition of DGUOK-AS1, miR-499a-5p, and SPRR1B was applied for exploring the biological function based on detection of cell viability, proliferation, migration, and apoptosis in CESC SiHa and HeLa cells. RESULTS: DGUOK-AS1 and SPRR1B expressions were obviously elevated, whereas the expression of miR-499a-5p was reduced in both CESC tissues and cells. Silencing of DGUOK-AS1 attenuated cell growth and boosted apoptosis of CESC cells. Notably, DGUOK-AS1 inhibited miR-499a-5p to release SPRR1B, which significantly accelerated the development of CESC. CONCLUSION: DGUOK-AS1sponging miR-499a-5p facilitated CESC cells progression by releasing SPRR1B in vitro. It provides a new sight for the treatment of CESC patients involving DGUOK-AS1-miR-499a-5p-SPRR1B.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Carcinoma de Células Escamosas / Regulación Neoplásica de la Expresión Génica / Neoplasias del Cuello Uterino / MicroARNs / Proteínas Ricas en Prolina del Estrato Córneo / ARN Largo no Codificante Límite: Female / Humans Idioma: En Revista: Biochem Biophys Res Commun Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Carcinoma de Células Escamosas / Regulación Neoplásica de la Expresión Génica / Neoplasias del Cuello Uterino / MicroARNs / Proteínas Ricas en Prolina del Estrato Córneo / ARN Largo no Codificante Límite: Female / Humans Idioma: En Revista: Biochem Biophys Res Commun Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos