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Arginine induces protein self-assembly into nanofibers for triggering osteogenic differentiation of stem cells.
Lei, Fang; Zhou, Guanshan; Chen, Yuping; Cai, Jiangfeng; Wang, Jie; Shuai, Yajun; Xu, Zongpu; Wang, Zhangfu; Mao, Chuanbin; Yang, Mingying.
Afiliación
  • Lei F; Institute of Applied Bioresource Research, College of Animal Science, Zhejiang University, Yuhangtang Road 866, Hangzhou 310058, China. yangm@zju.edu.cn.
  • Zhou G; Institute of Applied Bioresource Research, College of Animal Science, Zhejiang University, Yuhangtang Road 866, Hangzhou 310058, China. yangm@zju.edu.cn.
  • Chen Y; Institute of Applied Bioresource Research, College of Animal Science, Zhejiang University, Yuhangtang Road 866, Hangzhou 310058, China. yangm@zju.edu.cn.
  • Cai J; Institute of Applied Bioresource Research, College of Animal Science, Zhejiang University, Yuhangtang Road 866, Hangzhou 310058, China. yangm@zju.edu.cn.
  • Wang J; Institute of Applied Bioresource Research, College of Animal Science, Zhejiang University, Yuhangtang Road 866, Hangzhou 310058, China. yangm@zju.edu.cn.
  • Shuai Y; Institute of Applied Bioresource Research, College of Animal Science, Zhejiang University, Yuhangtang Road 866, Hangzhou 310058, China. yangm@zju.edu.cn.
  • Xu Z; Institute of Applied Bioresource Research, College of Animal Science, Zhejiang University, Yuhangtang Road 866, Hangzhou 310058, China. yangm@zju.edu.cn.
  • Wang Z; Department of Orthopaedics, Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical University, Linhai 317000, Zhejiang, China.
  • Mao C; Department of Chemistry & Biochemistry, Stephenson Life Science Research Center, Institute for Biomedical Engineering Science and Technology, University of Oklahoma, 101 Stephenson Parkway, Norman, Oklahoma 73019-5251, USA. maophage@gmail.com.
  • Yang M; School of Materials Science and Engineering, Zhejiang University, Hangzhou, Zhejiang 310027, P. R. China.
J Mater Chem B ; 9(47): 9764-9769, 2021 12 08.
Article en En | MEDLINE | ID: mdl-34806096
Although silk proteins are considered promising in building a scaffold for tissue engineering, one of the silk proteins, Bombyx mori silk sericin (BS), has limited processability in producing nanofibrous scaffolds because its surface charge anisotropy promotes gelation instead. To overcome this daunting challenge, we developed a mild and simple procedure for assembling BS into nanofibers and nanofibrous scaffolds. Briefly, arginine was added to the aqueous BS solution to reduce the negative charge of BS, thereby inducing BS to self-assemble into nanofibers in the solution. Circular dichroism (CD) and Fourier transform infrared (FT-IR) spectra showed that arginine promoted the formation of ß-sheet conformation in BS and increased its thermal stability. Furthermore, the arginine-induced BS nanofiber solution could be casted into scaffolds made of abundant network-like nanofibrous structures. The BS scaffolds promoted cell adhesion and growth and stimulated osteogenic differentiation of the bone marrow mesenchymal stem cells (BMSCs) in the absence of differentiation inducers in culture media. Our study presents a new strategy for assembling proteins into osteogenic nanofibrous scaffolds for inducing stem cell differentiation in regenerative medicine.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arginina / Diferenciación Celular / Sericinas / Andamios del Tejido / Nanofibras / Células Madre Mesenquimatosas Límite: Animals Idioma: En Revista: J Mater Chem B Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arginina / Diferenciación Celular / Sericinas / Andamios del Tejido / Nanofibras / Células Madre Mesenquimatosas Límite: Animals Idioma: En Revista: J Mater Chem B Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido