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Immunofluorescence Assay of Ablated Colorectal Liver Metastases: The Frozen Section of Image-Guided Tumor Ablation?
Vasiniotis Kamarinos, Nikiforos; Vakiani, Efsevia; Fujisawa, Sho; Gonen, Mithat; Fan, Ning; Romin, Yevgeniy; Do, Richard K G; Ziv, Etay; Erinjeri, Joseph P; Petre, Elena N; Sotirchos, Vlasios S; Camacho, Juan C; Solomon, Stephen B; Manova-Todorova, Katia; Sofocleous, Constantinos T.
Afiliación
  • Vasiniotis Kamarinos N; Interventional Oncology, IR Service, Department of Radiology, Memorial Sloan Kettering Cancer Center, New York, New York.
  • Vakiani E; Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, New York.
  • Fujisawa S; Department of Molecular Cytology, Memorial Sloan Kettering Cancer Center, New York, New York.
  • Gonen M; Department of Statistics, Memorial Sloan Kettering Cancer Center, New York, New York.
  • Fan N; Department of Molecular Cytology, Memorial Sloan Kettering Cancer Center, New York, New York.
  • Romin Y; Department of Molecular Cytology, Memorial Sloan Kettering Cancer Center, New York, New York.
  • Do RKG; Department of Radiology, Memorial Sloan Kettering Cancer Center, New York, New York.
  • Ziv E; Interventional Oncology, IR Service, Department of Radiology, Memorial Sloan Kettering Cancer Center, New York, New York.
  • Erinjeri JP; Interventional Oncology, IR Service, Department of Radiology, Memorial Sloan Kettering Cancer Center, New York, New York.
  • Petre EN; Interventional Oncology, IR Service, Department of Radiology, Memorial Sloan Kettering Cancer Center, New York, New York.
  • Sotirchos VS; Interventional Oncology, IR Service, Department of Radiology, Memorial Sloan Kettering Cancer Center, New York, New York.
  • Camacho JC; Interventional Oncology, IR Service, Department of Radiology, Memorial Sloan Kettering Cancer Center, New York, New York.
  • Solomon SB; Interventional Oncology, IR Service, Department of Radiology, Memorial Sloan Kettering Cancer Center, New York, New York.
  • Manova-Todorova K; Department of Molecular Cytology, Memorial Sloan Kettering Cancer Center, New York, New York.
  • Sofocleous CT; Interventional Oncology, IR Service, Department of Radiology, Memorial Sloan Kettering Cancer Center, New York, New York. Electronic address: sofoclec@mskcc.org.
J Vasc Interv Radiol ; 33(3): 308-315.e1, 2022 03.
Article en En | MEDLINE | ID: mdl-34800623
PURPOSE: To validate an immunofluorescence assay (IFA) detecting residual viable tumor (VT) as intraprocedural thermal ablation (TA) zone assessment and demonstrate its prognostic value for local tumor progression (LTP) after colorectal liver metastasis (CLM) TA. MATERIALS AND METHODS: This prospective study, approved by the institutional review board, included 99 patients with 155 CLMs ablated between November 2009 and January 2019. Tissue samples from the ablation zone (AZ) center and minimal margin underwent immunofluorescent microscopic examination interrogating cellular morphology and mitochondrial viability (IFA) within 30 minutes after ablation. The same tissue samples were subsequently evaluated with standard morphologic and immunohistochemical methods. The sensitivity, specificity, and overall accuracy of IFA versus standard morphologic and immunohistochemical examination were calculated. The LTP-free survival rates were evaluated for the 12-month follow-up period. RESULTS: Of the 311 tissue samples stained, 304 (98%) were deemed evaluable. Of these specimens, 27% (81/304) were considered positive for the presence of VT. The accuracy of IFA was 94% (286/304). The sensitivity and specificity were 100% (63/63) and 93% (223/241), respectively. The 18 false-positive IFA assessments corresponded to samples that included viable cholangiocytes. The 12-month LTP-free survival was 59% versus 78% for IFA positive versus negative for VT AZs, respectively (P < .001). There was no difference in LTP between margin positive only and central AZ-positive tumors (25% vs 31%, P = 1). CONCLUSIONS: The IFA assessment of the AZ can be completed intraprocedurally and serve as a valid real-time biomarker of complete tumor eradication or detect residual VT after TA. This method could improve tumor control by TA.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Colorrectales / Ablación por Catéter / Neoplasias Hepáticas Tipo de estudio: Observational_studies Límite: Humans Idioma: En Revista: J Vasc Interv Radiol Asunto de la revista: ANGIOLOGIA / RADIOLOGIA Año: 2022 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Colorrectales / Ablación por Catéter / Neoplasias Hepáticas Tipo de estudio: Observational_studies Límite: Humans Idioma: En Revista: J Vasc Interv Radiol Asunto de la revista: ANGIOLOGIA / RADIOLOGIA Año: 2022 Tipo del documento: Article Pais de publicación: Estados Unidos