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Diversity detected in commensals at host and farm level reveals implications for national antimicrobial resistance surveillance programmes.
Laird, Tanya J; Jordan, David; Lee, Zheng Zhou; O'Dea, Mark; Stegger, Marc; Truswell, Alec; Sahibzada, Shafi; Abraham, Rebecca; Abraham, Sam.
Afiliación
  • Laird TJ; Antimicrobial Resistance and Infectious Diseases Laboratory, College of Science, Health, Engineering and Education, Murdoch University, Murdoch, Western Australia, Australia.
  • Jordan D; NSW Department of Primary Industries, Wollongbar, New South Wales, Australia.
  • Lee ZZ; Antimicrobial Resistance and Infectious Diseases Laboratory, College of Science, Health, Engineering and Education, Murdoch University, Murdoch, Western Australia, Australia.
  • O'Dea M; Antimicrobial Resistance and Infectious Diseases Laboratory, College of Science, Health, Engineering and Education, Murdoch University, Murdoch, Western Australia, Australia.
  • Stegger M; DPIRD Diagnostic and Laboratory Services, South Perth, Western Australia, Australia.
  • Truswell A; Antimicrobial Resistance and Infectious Diseases Laboratory, College of Science, Health, Engineering and Education, Murdoch University, Murdoch, Western Australia, Australia.
  • Sahibzada S; Department of Bacteria, Parasites and Fungi, Statens Serum Institut, Copenhagen, Denmark.
  • Abraham R; Antimicrobial Resistance and Infectious Diseases Laboratory, College of Science, Health, Engineering and Education, Murdoch University, Murdoch, Western Australia, Australia.
  • Abraham S; Antimicrobial Resistance and Infectious Diseases Laboratory, College of Science, Health, Engineering and Education, Murdoch University, Murdoch, Western Australia, Australia.
J Antimicrob Chemother ; 77(2): 400-408, 2022 02 02.
Article en En | MEDLINE | ID: mdl-34791273
BACKGROUND: A key component to control of antimicrobial resistance (AMR) is the surveillance of food animals. Currently, national programmes test only limited isolates per animal species per year, an approach tacitly assuming that heterogeneity of AMR across animal populations is negligible. If the latter assumption is incorrect then the risk to humans from AMR in the food chain is underestimated. OBJECTIVES: To demonstrate the extent of phenotypic and genetic heterogeneity of Escherichia coli in swine to assess the need for improved protocols for AMR surveillance in food animals. METHODS: Eight E. coli isolates were obtained from each of 10 pigs on each of 10 farms. For these 800 isolates, AMR profiles (MIC estimates for six drugs) and PCR-based fingerprinting analysis were performed and used to select a subset (n = 151) for WGS. RESULTS: Heterogeneity in the phenotypic AMR traits of E. coli was observed in 89% of pigs, with 58% of pigs harbouring three or more distinct phenotypes. Similarly, 94% of pigs harboured two or more distinct PCR-fingerprinting profiles. Farm-level heterogeneity was detected, with ciprofloxacin resistance detected in only 60% of pigs from a single farm. Furthermore, 58 STs were identified, with the dominant STs being ST10, ST101, ST542 and ST641. CONCLUSIONS: Phenotypic and genotypic heterogeneity of AMR traits in bacteria from animal populations are real phenomena posing a barrier to correct interpretation of data from AMR surveillance. Evolution towards a more in-depth sampling model is needed to account for heterogeneity and increase the reliability of inferences.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Farmacorresistencia Bacteriana / Escherichia coli Tipo de estudio: Prognostic_studies / Screening_studies Límite: Animals Idioma: En Revista: J Antimicrob Chemother Año: 2022 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Farmacorresistencia Bacteriana / Escherichia coli Tipo de estudio: Prognostic_studies / Screening_studies Límite: Animals Idioma: En Revista: J Antimicrob Chemother Año: 2022 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Reino Unido