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Protein tyrosine phosphatase non-receptor type 12 (PTPN12), negatively regulated by miR-106a-5p, suppresses the progression of hepatocellular carcinoma.
Liang, Zhanqiang; Li, Xingxing; Duan, Fei; Song, Liming; Wang, Zhongzhen; Li, Xuemin; Yang, Pengsheng; Li, Liantao.
Afiliación
  • Liang Z; Department of Hepatobiliary Surgery, Zhengzhou Central Hospital Affiliated To Zhengzhou University, Zhengzhou, 450007, Henan, China.
  • Li X; Department of General Surgery, Xinzheng Public People's Hospital, Xinzheng, Zhengzhou, 451150, Henan, China.
  • Duan F; Department of Hepatobiliary Surgery, Zhengzhou Central Hospital Affiliated To Zhengzhou University, Zhengzhou, 450007, Henan, China.
  • Song L; Department of Hepatobiliary Surgery, Zhengzhou Central Hospital Affiliated To Zhengzhou University, Zhengzhou, 450007, Henan, China.
  • Wang Z; Department of General Surgery, Xinzheng Public People's Hospital, Xinzheng, Zhengzhou, 451150, Henan, China.
  • Li X; Department of Hepatobiliary Surgery, Zhengzhou Central Hospital Affiliated To Zhengzhou University, Zhengzhou, 450007, Henan, China.
  • Yang P; Department of Hepatobiliary Surgery, Zhengzhou Central Hospital Affiliated To Zhengzhou University, Zhengzhou, 450007, Henan, China.
  • Li L; Department of General Surgery, Xinzheng Public People's Hospital, Xinzheng, Zhengzhou, 451150, Henan, China. jio262352@163.com.
Hum Cell ; 35(1): 299-309, 2022 Jan.
Article en En | MEDLINE | ID: mdl-34784010
Protein tyrosine phosphatase non-receptor type 12 (PTPN12) is abnormally expressed in many human cancers. However, its role in hepatocellular carcinoma (HCC) is indeterminate. In this study, immunohistochemistry and Western blot were adopted to detect PTPN12 protein expression in HCC tissues and cell lines. MiR-106a-5p and PTPN12 mRNA expressions were determined by quantitative real-time polymerase chain reaction (qRT-PCR). siRNA was used to knockdown PTPN12 expression in HCC cells, and the multiplication, migration, and invasion of HCC cells were determined by cell counting kit 8 (CCK-8) and Transwell assays. The interaction between PTPN12 and miR-106a-5p was verified by dual-luciferase reporter gene assay and RNA immunoprecipitation (RIP) assay. In the present study, we demonstrated that PTPN12 expression in HCC tissues and cells was significantly decreased, which was associated with the tumor size, TNM stage, and lymph node metastasis of HCC patients. Functionally, knocking down PTPN12 significantly promoted the multiplication, migration, invasion, and epithelial-mesenchymal transition (EMT) of HCC cells. PTPN12 was identified as the direct target of miR-106a-5p, and its expression was negatively modulated by miR-106a-5p. Besides, PTPN12 counteracted the promoting effects of miR-106a-5p on the viability, migration, invasion, and EMT of HCC cells. In conclusion, this study substantiates that PTPN12 inhibits the growth, migration, invasion, and EMT of HCC cells, and miR-106a-5p contributes to its dysregulation in HCC.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Regulación Neoplásica de la Expresión Génica / Carcinoma Hepatocelular / MicroARNs / Proteína Tirosina Fosfatasa no Receptora Tipo 12 / Neoplasias Hepáticas Límite: Humans Idioma: En Revista: Hum Cell Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Japón
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Regulación Neoplásica de la Expresión Génica / Carcinoma Hepatocelular / MicroARNs / Proteína Tirosina Fosfatasa no Receptora Tipo 12 / Neoplasias Hepáticas Límite: Humans Idioma: En Revista: Hum Cell Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Japón