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Comparative Hessian Fly Larval Transcriptomics Provides Novel Insight into Host and Nonhost Resistance.
Subramanyam, Subhashree; Nemacheck, Jill A; Xie, Shaojun; Bhide, Ketaki; Thimmapuram, Jyothi; Scofield, Steven R; Sardesai, Nagesh.
Afiliación
  • Subramanyam S; Crop Production and Pest Control Research Unit, USDA-ARS, West Lafayette, IN 47907, USA.
  • Nemacheck JA; Department of Entomology, Purdue University, West Lafayette, IN 47907, USA.
  • Xie S; Crop Production and Pest Control Research Unit, USDA-ARS, West Lafayette, IN 47907, USA.
  • Bhide K; Department of Entomology, Purdue University, West Lafayette, IN 47907, USA.
  • Thimmapuram J; Bioinformatics Core, Purdue University, West Lafayette, IN 47907, USA.
  • Scofield SR; Bioinformatics Core, Purdue University, West Lafayette, IN 47907, USA.
  • Sardesai N; Bioinformatics Core, Purdue University, West Lafayette, IN 47907, USA.
Int J Mol Sci ; 22(21)2021 Oct 25.
Article en En | MEDLINE | ID: mdl-34768928
The Hessian fly is a destructive pest of wheat. Employing additional molecular strategies can complement wheat's native insect resistance. However, this requires functional characterization of Hessian-fly-responsive genes, which is challenging because of wheat genome complexity. The diploid Brachypodium distachyon (Bd) exhibits nonhost resistance to Hessian fly and displays phenotypic/molecular responses intermediate between resistant and susceptible host wheat, offering a surrogate genome for gene characterization. Here, we compared the transcriptomes of Biotype L larvae residing on resistant/susceptible wheat, and nonhost Bd plants. Larvae from susceptible wheat and nonhost Bd plants revealed similar molecular responses that were distinct from avirulent larval responses on resistant wheat. Secreted salivary gland proteins were strongly up-regulated in all larvae. Genes from various biological pathways and molecular processes were up-regulated in larvae from both susceptible wheat and nonhost Bd plants. However, Bd larval expression levels were intermediate between larvae from susceptible and resistant wheat. Most genes were down-regulated or unchanged in avirulent larvae, correlating with their inability to establish feeding sites and dying within 4-5 days after egg-hatch. Decreased gene expression in Bd larvae, compared to ones on susceptible wheat, potentially led to developmentally delayed 2nd-instars, followed by eventually succumbing to nonhost resistance defense mechanisms.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Triticum / Brachypodium / Resistencia a la Enfermedad / Nematocera Límite: Animals Idioma: En Revista: Int J Mol Sci Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Triticum / Brachypodium / Resistencia a la Enfermedad / Nematocera Límite: Animals Idioma: En Revista: Int J Mol Sci Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Suiza