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Computed structures of core eukaryotic protein complexes.
Humphreys, Ian R; Pei, Jimin; Baek, Minkyung; Krishnakumar, Aditya; Anishchenko, Ivan; Ovchinnikov, Sergey; Zhang, Jing; Ness, Travis J; Banjade, Sudeep; Bagde, Saket R; Stancheva, Viktoriya G; Li, Xiao-Han; Liu, Kaixian; Zheng, Zhi; Barrero, Daniel J; Roy, Upasana; Kuper, Jochen; Fernández, Israel S; Szakal, Barnabas; Branzei, Dana; Rizo, Josep; Kisker, Caroline; Greene, Eric C; Biggins, Sue; Keeney, Scott; Miller, Elizabeth A; Fromme, J Christopher; Hendrickson, Tamara L; Cong, Qian; Baker, David.
Afiliación
  • Humphreys IR; Department of Biochemistry, University of Washington, Seattle, WA, USA.
  • Pei J; Institute for Protein Design, University of Washington, Seattle, WA, USA.
  • Baek M; Eugene McDermott Center for Human Growth and Development, University of Texas Southwestern Medical Center, Dallas, TX, USA.
  • Krishnakumar A; Department of Biophysics, University of Texas Southwestern Medical Center, Dallas, TX, USA.
  • Anishchenko I; Department of Biochemistry, University of Washington, Seattle, WA, USA.
  • Ovchinnikov S; Institute for Protein Design, University of Washington, Seattle, WA, USA.
  • Zhang J; Department of Biochemistry, University of Washington, Seattle, WA, USA.
  • Ness TJ; Institute for Protein Design, University of Washington, Seattle, WA, USA.
  • Banjade S; Department of Biochemistry, University of Washington, Seattle, WA, USA.
  • Bagde SR; Institute for Protein Design, University of Washington, Seattle, WA, USA.
  • Stancheva VG; Faculty of Arts and Sciences, Division of Science, Harvard University, Cambridge, MA, USA.
  • Li XH; John Harvard Distinguished Science Fellowship Program, Harvard University, Cambridge, MA, USA.
  • Liu K; Eugene McDermott Center for Human Growth and Development, University of Texas Southwestern Medical Center, Dallas, TX, USA.
  • Zheng Z; Department of Biophysics, University of Texas Southwestern Medical Center, Dallas, TX, USA.
  • Barrero DJ; Department of Chemistry, Wayne State University, Detroit, MI, USA.
  • Roy U; Department of Molecular Biology and Genetics, Weill Institute for Cell and Molecular Biology, Cornell University, Ithaca, NY, USA.
  • Kuper J; Department of Molecular Biology and Genetics, Weill Institute for Cell and Molecular Biology, Cornell University, Ithaca, NY, USA.
  • Fernández IS; MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK.
  • Szakal B; MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK.
  • Branzei D; Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA.
  • Rizo J; Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA.
  • Kisker C; Gerstner Sloan Kettering Graduate School of Biomedical Sciences, New York, NY, USA.
  • Greene EC; Howard Hughes Medical Institute, Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA, USA.
  • Biggins S; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA.
  • Keeney S; Rudolf Virchow Center for Integrative and Translational Bioimaging, University of Würzburg, Würzburg, Germany.
  • Miller EA; Department of Structural Biology, St Jude Children's Research Hospital, Memphis, TN, USA.
  • Fromme JC; IFOM, the FIRC Institute of Molecular Oncology, Via Adamello 16, 20139, Milan, Italy.
  • Hendrickson TL; IFOM, the FIRC Institute of Molecular Oncology, Via Adamello 16, 20139, Milan, Italy.
  • Cong Q; Istituto di Genetica Molecolare, Consiglio Nazionale delle Ricerche (IGM-CNR), Via Abbiategrasso 207, 27100, Pavia, Italy.
  • Baker D; Department of Biophysics, University of Texas Southwestern Medical Center, Dallas, TX, USA.
Science ; 374(6573): eabm4805, 2021 Dec 10.
Article en En | MEDLINE | ID: mdl-34762488
Protein-protein interactions play critical roles in biology, but the structures of many eukaryotic protein complexes are unknown, and there are likely many interactions not yet identified. We take advantage of advances in proteome-wide amino acid coevolution analysis and deep-learning­based structure modeling to systematically identify and build accurate models of core eukaryotic protein complexes within the Saccharomyces cerevisiae proteome. We use a combination of RoseTTAFold and AlphaFold to screen through paired multiple sequence alignments for 8.3 million pairs of yeast proteins, identify 1505 likely to interact, and build structure models for 106 previously unidentified assemblies and 806 that have not been structurally characterized. These complexes, which have as many as five subunits, play roles in almost all key processes in eukaryotic cells and provide broad insights into biological function.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteoma / Proteínas de Saccharomyces cerevisiae / Mapeo de Interacción de Proteínas / Complejos Multiproteicos / Aprendizaje Profundo Tipo de estudio: Prognostic_studies Idioma: En Revista: Science Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteoma / Proteínas de Saccharomyces cerevisiae / Mapeo de Interacción de Proteínas / Complejos Multiproteicos / Aprendizaje Profundo Tipo de estudio: Prognostic_studies Idioma: En Revista: Science Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos