Your browser doesn't support javascript.
loading
Construction of an Integrated Device of a Self-Powered Biosensor and Matching Capacitor Based on Graphdiyne and Multiple Signal Amplification: Ultrasensitive Method for MicroRNA Detection.
Hou, Yang-Yang; Xu, Jing; Wang, Fu-Ting; Dong, Zhong; Tan, Xuecai; Huang, Ke-Jing; Li, Jia-Qiang; Zuo, Chun-Yang; Zhang, Si-Qi.
Afiliación
  • Hou YY; College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China.
  • Xu J; College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China.
  • Wang FT; College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China.
  • Dong Z; College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China.
  • Tan X; Key Laboratory of Chemistry and Engineering of Forest Products, Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, Key Laboratory of Guangxi Colleges and Universities for Food Safety and Pharmaceutical Analytical Chemistry, School of Chemistry and Chemical and Engineering, Guang
  • Huang KJ; College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China.
  • Li JQ; Key Laboratory of Chemistry and Engineering of Forest Products, Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, Key Laboratory of Guangxi Colleges and Universities for Food Safety and Pharmaceutical Analytical Chemistry, School of Chemistry and Chemical and Engineering, Guang
  • Zuo CY; Advanced Membranes and Porous Materials Center, Physical Sciences and Engineering Division, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia.
  • Zhang SQ; College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China.
Anal Chem ; 93(46): 15225-15230, 2021 11 23.
Article en En | MEDLINE | ID: mdl-34752059
The detection of microRNA (miRNA) in human serum has great significance for cancer prevention. Herein, a novel self-powered biosensing platform is developed, which effectively integrates an enzymatic biofuel cell (EBFC)-based self-powered biosensor with a matching capacitor for miRNA detection. A catalytic hairpin assembly and hybrid chain reaction are used to improve the analytical performance of EBFC. Furthermore, the matching capacitor is selected as an auxiliary signal amplifying device, and graphdiyne is applied as substrate material for EBFC. The results confirm that the developed method obviously increases the output current of EBFC, and the sensitivity can reach 2.75 µA/pM, which is 786% of pure EBFC. MiRNA can be detected in an expanded linear range of 0.1-100000 fM with a detection limit of 0.034 fM (S/N = 3). It can offer a selective and sensitive platform for nucleotide sequence detection with great potential in clinical diagnostics.
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles / MicroARNs Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Anal Chem Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles / MicroARNs Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Anal Chem Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos