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RNA promotes the formation of spatial compartments in the nucleus.
Quinodoz, Sofia A; Jachowicz, Joanna W; Bhat, Prashant; Ollikainen, Noah; Banerjee, Abhik K; Goronzy, Isabel N; Blanco, Mario R; Chovanec, Peter; Chow, Amy; Markaki, Yolanda; Thai, Jasmine; Plath, Kathrin; Guttman, Mitchell.
Afiliación
  • Quinodoz SA; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
  • Jachowicz JW; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
  • Bhat P; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA; David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA.
  • Ollikainen N; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
  • Banerjee AK; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA; Keck School of Medicine, University of Southern California, Los Angeles, CA 90089, USA.
  • Goronzy IN; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA; David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA.
  • Blanco MR; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
  • Chovanec P; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
  • Chow A; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
  • Markaki Y; Department of Biological Chemistry, University of California, Los Angeles, Los Angeles, CA 90095, USA.
  • Thai J; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
  • Plath K; Department of Biological Chemistry, University of California, Los Angeles, Los Angeles, CA 90095, USA.
  • Guttman M; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA. Electronic address: mguttman@caltech.edu.
Cell ; 184(23): 5775-5790.e30, 2021 11 11.
Article en En | MEDLINE | ID: mdl-34739832
RNA, DNA, and protein molecules are highly organized within three-dimensional (3D) structures in the nucleus. Although RNA has been proposed to play a role in nuclear organization, exploring this has been challenging because existing methods cannot measure higher-order RNA and DNA contacts within 3D structures. To address this, we developed RNA & DNA SPRITE (RD-SPRITE) to comprehensively map the spatial organization of RNA and DNA. These maps reveal higher-order RNA-chromatin structures associated with three major classes of nuclear function: RNA processing, heterochromatin assembly, and gene regulation. These data demonstrate that hundreds of ncRNAs form high-concentration territories throughout the nucleus, that specific RNAs are required to recruit various regulators into these territories, and that these RNAs can shape long-range DNA contacts, heterochromatin assembly, and gene expression. These results demonstrate a mechanism where RNAs form high-concentration territories, bind to diffusible regulators, and guide them into compartments to regulate essential nuclear functions.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ARN / Núcleo Celular Límite: Animals / Female / Humans Idioma: En Revista: Cell Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ARN / Núcleo Celular Límite: Animals / Female / Humans Idioma: En Revista: Cell Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos