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Insulin in the saliva of pigs: Validation of an automated assay and changes at different physiological conditions.
Ortín-Bustillo, A; Tvarijonaviciute, A; Cerón, J J; Contreras-Aguilar, M D; Martínez-Miró, S; Martínez-Subiela, S; Tecles, F; Muñoz-Prieto, A.
Afiliación
  • Ortín-Bustillo A; Interdisciplinary Laboratory of Clinical Analysis (Interlab-UMU), Veterinary School, Campus of Excellence Mare Nostrum, University of Murcia, Espinardo, 30100 Murcia, Spain.
  • Tvarijonaviciute A; Interdisciplinary Laboratory of Clinical Analysis (Interlab-UMU), Veterinary School, Campus of Excellence Mare Nostrum, University of Murcia, Espinardo, 30100 Murcia, Spain.
  • Cerón JJ; Interdisciplinary Laboratory of Clinical Analysis (Interlab-UMU), Veterinary School, Campus of Excellence Mare Nostrum, University of Murcia, Espinardo, 30100 Murcia, Spain.
  • Contreras-Aguilar MD; Interdisciplinary Laboratory of Clinical Analysis (Interlab-UMU), Veterinary School, Campus of Excellence Mare Nostrum, University of Murcia, Espinardo, 30100 Murcia, Spain.
  • Martínez-Miró S; Department of Animal Production, Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, Campus de Espinardo s/n, 30100 Espinardo, Murcia, Spain.
  • Martínez-Subiela S; Interdisciplinary Laboratory of Clinical Analysis (Interlab-UMU), Veterinary School, Campus of Excellence Mare Nostrum, University of Murcia, Espinardo, 30100 Murcia, Spain.
  • Tecles F; Interdisciplinary Laboratory of Clinical Analysis (Interlab-UMU), Veterinary School, Campus of Excellence Mare Nostrum, University of Murcia, Espinardo, 30100 Murcia, Spain. Electronic address: ftecles@um.es.
  • Muñoz-Prieto A; Clinic for Internal Diseases, Faculty of Veterinary Medicine, University of Zagreb, Hiezelova 55, 10000 Zagreb, Croatia.
Res Vet Sci ; 141: 110-115, 2021 Dec.
Article en En | MEDLINE | ID: mdl-34715588
This study aimed to evaluate whether insulin could be measured in the saliva of pigs and if its concentration changes in some physiological conditions. For this purpose, a validation of an automated heterologous immunoassay for measuring insulin in the saliva of pigs was performed. In addition, the possible changes of salivary insulin concentration in sows after food intake and during gestation and lactation were studied. The evaluated immunoassay was able to detect insulin in the saliva of pigs in a precise and accurate way when species-specific calibrators were used. There was no correlation in insulin concentrations between serum and saliva. Insulin concentrations showed a significant increase in the saliva of sows after feeding. Sows at farrowing and lactation presented higher salivary insulin levels as compared with those in gestation. In conclusion, the results showed that insulin could be measured in the saliva of pigs, and changes in its concentration can be detected due to food intake and different physiological conditions.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Saliva / Insulina Límite: Animals Idioma: En Revista: Res Vet Sci Año: 2021 Tipo del documento: Article País de afiliación: España Pais de publicación: Reino Unido
Texto completo
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XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Saliva / Insulina Límite: Animals Idioma: En Revista: Res Vet Sci Año: 2021 Tipo del documento: Article País de afiliación: España Pais de publicación: Reino Unido