Your browser doesn't support javascript.
loading
Expression of degQ gene and its effect on lipopeptide production as well as formation of secretory proteases in Bacillus subtilis strains.
Lilge, Lars; Vahidinasab, Maliheh; Adiek, Isabel; Becker, Philipp; Kuppusamy Nesamani, Chanthiya; Treinen, Chantal; Hoffmann, Mareen; Morabbi Heravi, Kambiz; Henkel, Marius; Hausmann, Rudolf.
Afiliación
  • Lilge L; Department of Bioprocess Engineering (150k), Institute of Food Science and Biotechnology (150), University of Hohenheim, Stuttgart, Germany.
  • Vahidinasab M; Department of Bioprocess Engineering (150k), Institute of Food Science and Biotechnology (150), University of Hohenheim, Stuttgart, Germany.
  • Adiek I; Department of Bioprocess Engineering (150k), Institute of Food Science and Biotechnology (150), University of Hohenheim, Stuttgart, Germany.
  • Becker P; Department of Bioprocess Engineering (150k), Institute of Food Science and Biotechnology (150), University of Hohenheim, Stuttgart, Germany.
  • Kuppusamy Nesamani C; Department of Bioprocess Engineering (150k), Institute of Food Science and Biotechnology (150), University of Hohenheim, Stuttgart, Germany.
  • Treinen C; Department of Bioprocess Engineering (150k), Institute of Food Science and Biotechnology (150), University of Hohenheim, Stuttgart, Germany.
  • Hoffmann M; Department of Bioprocess Engineering (150k), Institute of Food Science and Biotechnology (150), University of Hohenheim, Stuttgart, Germany.
  • Morabbi Heravi K; Department of Bioprocess Engineering (150k), Institute of Food Science and Biotechnology (150), University of Hohenheim, Stuttgart, Germany.
  • Henkel M; Department of Bioprocess Engineering (150k), Institute of Food Science and Biotechnology (150), University of Hohenheim, Stuttgart, Germany.
  • Hausmann R; Department of Bioprocess Engineering (150k), Institute of Food Science and Biotechnology (150), University of Hohenheim, Stuttgart, Germany.
Microbiologyopen ; 10(5): e1241, 2021 10.
Article en En | MEDLINE | ID: mdl-34713601
Bacillus subtilis is described as a promising production strain for lipopeptides. In the case of B. subtilis strains JABs24 and DSM10T , surfactin and plipastatin are produced. Lipopeptide formation is controlled, among others, by the DegU response regulator. The activating phospho-transfer by the DegS sensor kinase is stimulated by the pleiotropic regulator DegQ, resulting in enhanced DegU activation. In B. subtilis 168, a point mutation in the degQ promoter region leads to a reduction in gene expression. Corresponding reporter strains showed a 14-fold reduced expression. This effect on degQ expression and the associated impact on lipopeptide formation was examined for B. subtilis JABs24, a lipopeptide-producing derivative of strain 168, and B. subtilis wild-type strain DSM10T , which has a native degQ expression. Based on the stimulatory effects of the DegU regulator on secretory protease formation, the impact of degQ expression on extracellular protease activity was additionally investigated. To follow the impact of degQ, a deletion mutant was constructed for DSM10T , while a natively expressed degQ version was integrated into strain JABs24. This allowed strain-specific quantification of the stimulatory effect of degQ expression on plipastatin and the negative effect on surfactin production in strains JABs24 and DSM10T . While an unaffected degQ expression reduced surfactin production in JABs24 by about 25%, a sixfold increase in plipastatin was observed. In contrast, degQ deletion in DSM10T increased surfactin titer by threefold but decreased plipastatin production by fivefold. In addition, although significant differences in extracellular protease activity were detected, no decrease in plipastatin and surfactin produced during cultivation was observed.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oligopéptidos / Péptido Hidrolasas / Péptidos Cíclicos / Bacillus subtilis / Proteínas Bacterianas / Transactivadores / Lipopéptidos / Ácidos Grasos Idioma: En Revista: Microbiologyopen Año: 2021 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oligopéptidos / Péptido Hidrolasas / Péptidos Cíclicos / Bacillus subtilis / Proteínas Bacterianas / Transactivadores / Lipopéptidos / Ácidos Grasos Idioma: En Revista: Microbiologyopen Año: 2021 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido