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A standardized extract of Centella asiatica (ECa 233) prevents temporomandibular joint osteoarthritis by modulating the expression of local inflammatory mediators in mice.
Rotpenpian, Nattapon; Arayapisit, Tawepong; Roumwong, Atitaya; Pakaprot, Narawut; Tantisira, Mayuree; Wanasuntronwong, Aree.
Afiliación
  • Rotpenpian N; Faculty of Dentistry, Prince of Songkla University, Songkhla, Thailand.
  • Arayapisit T; Faculty of Dentistry, Mahidol University, Bangkok, Thailand.
  • Roumwong A; Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
  • Pakaprot N; Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand.
  • Tantisira M; Faculty of Pharmaceutical Sciences, Burapha University, Chonburi, Thailand.
  • Wanasuntronwong A; Faculty of Dentistry, Mahidol University, Bangkok, Thailand.
J Appl Oral Sci ; 29: e20210329, 2021.
Article en En | MEDLINE | ID: mdl-34705985
OBJECTIVES: To investigate the effect of a standardized extract of Centella asiatica (ECa 233), which has anti-inflammatory properties, on the local expression of the transient receptor potential vanilloid 1 (TRPV1), the acid-sensing ion channel subunit 3 (ASIC3), and the calcitonin gene-related peptide (CGRP) in the temporomandibular joint (TMJ) structure 21 days after injecting the TMJ with complete Freund's adjuvant (CFA). METHODOLOGY: A mouse model was induced by analyzing the CFA-injected TMJ on days 7, 14, and 21. We assessed TMJ histology by the osteoarthritis cartilage grade score. Then, we observed the effect of different ECa 233 concentrations (30, 100, and 300 mg/kg) and of 140 mg/kg ibuprofen doses on TRPV1, ASIC3, and CGRP local expression on day 21. RESULTS: Osteoarthritis cartilage scores were 1.17±0.37 and 3.83±0.68 on days 14 and 21, respectively, in the CFA group (n=5). On day 21, TRPV1, ASIC3, and CGRP expression significantly increased in the CFA group. In the ibuprofen-treated group, TRPV1 expression significantly decreased, but ASIC3 and CGRP showed no significant difference. All ECa 233 doses reduced TRPV1 expression, but the 100 mg/kg ECa 233 dose significantly decreased ASIC3 expression. CONCLUSIONS: TRPV1, ASIC3, and CGRP expression increased in mice with TMJ-OA on day 21. All ECa 233 and ibuprofen doses inhibited pathogenesis by modulating the local expression of TRPV1 and ASIC3. Therefore, ECa 233 was more effective than ibuprofen.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteoartritis / Centella Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J Appl Oral Sci Asunto de la revista: ODONTOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: Tailandia Pais de publicación: Brasil
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteoartritis / Centella Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J Appl Oral Sci Asunto de la revista: ODONTOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: Tailandia Pais de publicación: Brasil