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Excess polyspermy reduces the ability of porcine oocytes to promote male pronuclear formation after in vitro fertilization.
Nguyen, Hiep Thi; Dang-Nguyen, Thanh Quang; Somfai, Tamas; Men, Nguyen Thi; Beck-Woerner, Barbara; Viet Linh, Nguyen; Xuan Nguyen, Bui; Noguchi, Junko; Kaneko, Hiroyuki; Kikuchi, Kazuhiro.
Afiliación
  • Nguyen HT; Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Tsukuba, Japan.
  • Dang-Nguyen TQ; The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan.
  • Somfai T; Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam.
  • Men NT; Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Tsukuba, Japan.
  • Beck-Woerner B; Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Tsukuba, Japan.
  • Viet Linh N; Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Tsukuba, Japan.
  • Xuan Nguyen B; Life Sciences and Facility Management, Zurich University of Applied Sciences, Wädenswil, Switzerland.
  • Noguchi J; Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam.
  • Kaneko H; Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam.
  • Kikuchi K; Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Tsukuba, Japan.
Anim Sci J ; 92(1): e13650, 2021.
Article en En | MEDLINE | ID: mdl-34697861
Male pronucleus (MPN) formation is a very important physiological event during fertilization, which affects in vitro production of transferrable embryos. The aim of this study was to find out the correlation between the number of penetrated sperm and the occurrence of failure of MPN formation in porcine oocytes. In vitro matured porcine oocytes were fertilized in vitro with frozen epididymal sperm. Two different frozen sperm lots were tested in this study, which were different in terms of polyspermy rates. The numbers and the status of penetrated sperm in oocytes were evaluated 10 h after insemination. Under high polyspermy condition, the polyspermy rate was 83.5% with an average mean of 3.5 sperms per penetrated oocyte, whereas the percentage of polyspermy was 65.5% with an average mean of 2.4 sperms per penetrated oocyte under moderate polyspermic condition. Correlation analysis revealed a negative correlation between the number of penetrated sperm and their MPN formation percentage both in the sperm lot of high polyspermy (R = -0.560, p < 0.05) and in the sperm lot of moderate polyspermy (R = -0.405, p < 0.05) which suggests that penetration of excessive spermatozoa disables the oocyte cytoplasm to promote MPN formation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Interacciones Espermatozoide-Óvulo / Fertilización In Vitro Límite: Animals Idioma: En Revista: Anim Sci J Asunto de la revista: MEDICINA VETERINARIA Año: 2021 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Australia
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Interacciones Espermatozoide-Óvulo / Fertilización In Vitro Límite: Animals Idioma: En Revista: Anim Sci J Asunto de la revista: MEDICINA VETERINARIA Año: 2021 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Australia