Application of the 3' mRNA-Seq using unique molecular identifiers in highly degraded RNA derived from formalin-fixed, paraffin-embedded tissue.

Jang, Jin Sung; Holicky, Eileen; Lau, Julie; McDonough, Samantha; Mutawe, Mark; Koster, Matthew J; Warrington, Kenneth J; Cuninngham, Julie M

Jang, Jin Sung; Holicky, Eileen; Lau, Julie; McDonough, Samantha; Mutawe, Mark; Koster, Matthew J; Warrington, Kenneth J; Cuninngham, Julie M.

Afiliación

Jang JS; Genome Analysis Core, Medical Genome Facility, Center for Individualized Medicine, Mayo Clinic, Stabile Research Building, 200 First Street SW, Rochester, MN, 55905, USA. jang.jin@mayo.edu.
Holicky E; Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA. jang.jin@mayo.edu.
Lau J; Genome Analysis Core, Medical Genome Facility, Center for Individualized Medicine, Mayo Clinic, Stabile Research Building, 200 First Street SW, Rochester, MN, 55905, USA.
McDonough S; Genome Analysis Core, Medical Genome Facility, Center for Individualized Medicine, Mayo Clinic, Stabile Research Building, 200 First Street SW, Rochester, MN, 55905, USA.
Mutawe M; Genome Analysis Core, Medical Genome Facility, Center for Individualized Medicine, Mayo Clinic, Stabile Research Building, 200 First Street SW, Rochester, MN, 55905, USA.
Koster MJ; Genome Analysis Core, Medical Genome Facility, Center for Individualized Medicine, Mayo Clinic, Stabile Research Building, 200 First Street SW, Rochester, MN, 55905, USA.
Warrington KJ; Department of Internal Medicine, Division of Rheumatology, Mayo Clinic, Rochester, MN, USA.
Cuninngham JM; Department of Internal Medicine, Division of Rheumatology, Mayo Clinic, Rochester, MN, USA.

BMC Genomics ; 22(1): 759, 2021 Oct 24.

Article en En | MEDLINE | ID: mdl-34689749

Asunto(s)

Perfilación de la Expresión Génica; ARN; Formaldehído; Humanos; Adhesión en Parafina; ARN Mensajero/genética; Análisis de Secuencia de ARN; Fijación del Tejido

Palabras clave

3' mRNA-Seq; FFPE; Gene expression; PCR amplification bias; UMI

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ARN / Perfilación de la Expresión Génica Límite: Humans Idioma: En Revista: BMC Genomics Asunto de la revista: GENETICA Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido

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