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Leukocyte and cytokine variables in asymptomatic Pugs at genetic risk of necrotizing meningoencephalitis.
Windsor, Rebecca; Stewart, Samuel D; Talboom, Joshua; Lewis, Candace; Naymik, Marcus; Piras, Ignazio S; Keller, Stefan; Borjesson, Dori L; Clark, Gary; Khanna, Chand; Huentelman, Matthew.
Afiliación
  • Windsor R; Ethos Veterinary Health, Woburn, Massachusetts, USA.
  • Stewart SD; Ethos Discovery (501c3), San Diego, California, USA.
  • Talboom J; Ethos Veterinary Health, Woburn, Massachusetts, USA.
  • Lewis C; Ethos Discovery (501c3), San Diego, California, USA.
  • Naymik M; Neurogenomics Division, Translational Genomics Research Institute, Phoenix, Arizona, USA.
  • Piras IS; Neurogenomics Division, Translational Genomics Research Institute, Phoenix, Arizona, USA.
  • Keller S; Neurogenomics Division, Translational Genomics Research Institute, Phoenix, Arizona, USA.
  • Borjesson DL; Neurogenomics Division, Translational Genomics Research Institute, Phoenix, Arizona, USA.
  • Clark G; Department of Pathology, Microbiology, Immunology, University of California, Davis, Davis, California, USA.
  • Khanna C; Department of Pathology, Microbiology, Immunology, University of California, Davis, Davis, California, USA.
  • Huentelman M; Gary Clark Statistical Consulting LLC, Superior, Colorado, USA.
J Vet Intern Med ; 35(6): 2846-2852, 2021 Nov.
Article en En | MEDLINE | ID: mdl-34687084
BACKGROUND: Necrotizing meningoencephalitis (NME, aka Pug dog encephalitis) is an inflammatory brain condition associated with advanced disease at initial presentation, rapid progression, and poor response to conventional immunomodulatory therapy. HYPOTHESIS/OBJECTIVES: That genetic risk for NME, defined by a common germline DNA haplotype located on chromosome 12, is associated with altered blood cytokine concentrations and leukocyte subsets in asymptomatic Pugs. ANIMALS: Forty Pug dogs asymptomatic for NME from a hospital sample. METHODS: Prospective observational cohort study, including germline genome-wide genotyping, plasma cytokine determination by multiplexed profiling, and leukocyte subset characterization by flow cytometric analysis. RESULTS: Seven (18%) dogs were high risk, 10 (25%) medium risk, and 23 (58%) low risk for NME, giving a risk haplotype frequency of 30%. High and medium risk Pugs had significantly lower proportion of CD4+ T cells (median 22% [range, 7.3%-38%] vs 29% [range, 16%-41%], P = .03) and higher plasma IL-10 concentrations than low-risk Pugs (median 14.11 pg/mL [range, 9.66-344.19 pg/mL] vs 12.21 pg/mL [range, 2.59-18.53 pg/mL], P = .001). No other variables were significantly associated with the NME haplotype-based risk. CONCLUSIONS AND CLINICAL IMPORTANCE: These data suggest an immunological underpinning to NME and a biologic rationale for future clinical trials that investigate novel diagnostic, preventative, and therapeutic strategies for this disease.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de los Perros / Meningoencefalitis Tipo de estudio: Etiology_studies / Observational_studies / Risk_factors_studies Límite: Animals Idioma: En Revista: J Vet Intern Med Asunto de la revista: MEDICINA INTERNA / MEDICINA VETERINARIA Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de los Perros / Meningoencefalitis Tipo de estudio: Etiology_studies / Observational_studies / Risk_factors_studies Límite: Animals Idioma: En Revista: J Vet Intern Med Asunto de la revista: MEDICINA INTERNA / MEDICINA VETERINARIA Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos