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A Cold-Shock Protein from the South Pole-Dwelling Soil Bacterium Arthrobacter sp. Confers Cold Tolerance to Rice.
Kim, So Young; Kim, Joung Sug; Cho, Woosuk; Jun, Kyong Mi; Du, Xiaoxuan; Kim, Kyung Do; Kim, Yeon-Ki; Lee, Gang-Seob.
Afiliación
  • Kim SY; Biosafety Division, National Institute of Agricultural Sciences, Jeonju 54874, Korea.
  • Kim JS; Department of Biosciences and Bioinformatics, Myongji University, 116 Myongji-ro, Cheoin-gu, Yongin 17060, Korea.
  • Cho W; Biosafety Division, National Institute of Agricultural Sciences, Jeonju 54874, Korea.
  • Jun KM; Genomics Genetics Institute, GreenGene BioTech, Inc., 16-4 Dongbaek jungang-ro 16beon-gil, Giheung-gu, Yongin 17015, Korea.
  • Du X; Biosafety Division, National Institute of Agricultural Sciences, Jeonju 54874, Korea.
  • Kim KD; Department of Biosciences and Bioinformatics, Myongji University, 116 Myongji-ro, Cheoin-gu, Yongin 17060, Korea.
  • Kim YK; Department of Biosciences and Bioinformatics, Myongji University, 116 Myongji-ro, Cheoin-gu, Yongin 17060, Korea.
  • Lee GS; Biosafety Division, National Institute of Agricultural Sciences, Jeonju 54874, Korea.
Genes (Basel) ; 12(10)2021 10 09.
Article en En | MEDLINE | ID: mdl-34680989
Low temperature is a critical environmental factor restricting the physiology of organisms across kingdoms. In prokaryotes, cold shock induces the expression of various genes and proteins involved in cellular processes. Here, a cold-shock protein (ArCspA) from the South Pole-dwelling soil bacterium Arthrobacter sp. A2-5 was introduced into rice, a monocot model plant species. Four-week-old 35S:ArCspA transgenic rice plants grown in a cold chamber at 4 °C survived for 6 days. Cold stress significantly decreased the chlorophyll content in WT plants after 4 days compared with that in 35S:ArCspA transgenic plants. RNA-seq analysis was performed on WT and 35S:ArCspA transgenic rice with/without cold stress. GO terms such as "response to stress (GO:0006950)", "response to cold (GO:0009409)", and "response to heat (GO:0009408)" were significantly enriched among the upregulated genes in the 35S:ArCspA transgenic rice under normal conditions, even without cold-stress treatment. The expression of five cold stress-related genes, Rab16B (Os11g0454200), Rab21 (Os11g0454300), LEA22 (Os01g0702500), ABI5 (Os01 g0859300), and MAPK5 (Os03g0285800), was significantly upregulated in the transgenic rice compared with the WT rice. These results indicate that the ArCspA gene might be involved in the induction of cold-responsive genes and provide cold tolerance.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arthrobacter / Oryza / Microbiología del Suelo / Adaptación Fisiológica / Frío / Proteínas y Péptidos de Choque por Frío Tipo de estudio: Prognostic_studies Idioma: En Revista: Genes (Basel) Año: 2021 Tipo del documento: Article Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arthrobacter / Oryza / Microbiología del Suelo / Adaptación Fisiológica / Frío / Proteínas y Péptidos de Choque por Frío Tipo de estudio: Prognostic_studies Idioma: En Revista: Genes (Basel) Año: 2021 Tipo del documento: Article Pais de publicación: Suiza