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Detection of Plasmodium Sporozoites in Anopheles Mosquitoes using an Enzyme-linked Immunosorbent Assay.
Kumpitak, Chalermpon; Nguitragool, Wang; Cui, Liwang; Sattabongkot, Jetsumon; Bantuchai, Sirasate.
Afiliación
  • Kumpitak C; Mahidol Vivax Research Unit, Faculty of Tropical Medicine, Mahidol University.
  • Nguitragool W; Department of Molecular Tropical Medicine & Genetics, Faculty of Tropical Medicine, Mahidol University.
  • Cui L; Department of Internal Medicine, Morsani College of Medicine, University of South Florida.
  • Sattabongkot J; Mahidol Vivax Research Unit, Faculty of Tropical Medicine, Mahidol University.
  • Bantuchai S; Mahidol Vivax Research Unit, Faculty of Tropical Medicine, Mahidol University; sirasate.ban@mahidol.ac.th.
J Vis Exp ; (175)2021 09 30.
Article en En | MEDLINE | ID: mdl-34661581
Plasmodium sporozoites are the infective stage of malaria parasites that infect humans. The sporozoites residing in the salivary glands of female Anopheles mosquitoes are transmitted to humans via mosquito bites during blood feeding. The presence of sporozoites in the mosquito salivary glands thus defines mosquito infectiousness. To determine whether an Anopheles mosquito carries Plasmodium sporozoites, the enzyme-linked immunosorbent assay (ELISA) method has been the standard tool to detect the Plasmodium circumsporozoite protein (CSP), the major surface protein of the sporozoites. In this method, the head along with the thorax of each mosquito is separated from the abdomen, homogenized, and subjected to a sandwich ELISA to detect the presence of CSP specific to Plasmodium falciparum and each of the two subtypes, VK210 and VK247, of Plasmodium vivax.This method has been used to study malaria transmission, including the seasonal dynamics of mosquito infection and the species of the major malaria vectors in the study sites.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plasmodium / Anopheles Tipo de estudio: Diagnostic_studies Límite: Animals / Female / Humans Idioma: En Revista: J Vis Exp Año: 2021 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plasmodium / Anopheles Tipo de estudio: Diagnostic_studies Límite: Animals / Female / Humans Idioma: En Revista: J Vis Exp Año: 2021 Tipo del documento: Article Pais de publicación: Estados Unidos