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Improved production of deglucosylated platycodin D from saponins from balloon flower leaf by a food-grade enzyme using high hydrostatic pressure.
Shin, Kyung-Chul; Kim, Dae Wook; Oh, Yu Jin; Seo, Min-Ju; Na, Chae Sun; Kim, Yeong-Su.
Afiliación
  • Shin KC; Department of Integrative Bioscience and Biotechnology, Konkuk University, Seoul, 05029, Republic of Korea.
  • Kim DW; Department of Wild Plants and Seeds Conservation, Baekdudaegan National Arboretum, Bonghwa, 36209, Republic of Korea.
  • Oh YJ; Department of Wild Plants and Seeds Conservation, Baekdudaegan National Arboretum, Bonghwa, 36209, Republic of Korea.
  • Seo MJ; Department of Biochemistry, Molecular Biology & Biophysics, University of Minnesota, 140 Gortner Laboratory, 1479 Gortner Avenue, Saint Paul, MN, 55108, USA.
  • Na CS; Department of Wild Plants and Seeds Conservation, Baekdudaegan National Arboretum, Bonghwa, 36209, Republic of Korea.
  • Kim YS; Department of Wild Plants and Seeds Conservation, Baekdudaegan National Arboretum, Bonghwa, 36209, Republic of Korea.
Heliyon ; 7(10): e08104, 2021 Oct.
Article en En | MEDLINE | ID: mdl-34660923
Platycosides, saponins contained in balloon flower, which have been used as food health supplements for respiratory diseases, have diverse pharmacological effects. Platycosides exhibit better pharmacological activity by hydrolyzing their own sugars. However, to date, there have been no studies on the production of deglucosylated platycodin D suitable for food applications. In this study, Pluszyme 2000P, which was derived from Aspergillus niger, a food-grade microorganism, was used to completely convert platycoside E into deglucosylated platycodin D. For an efficient and economical production of deglucosylated platycodin D, the productivity was improved approximately 2.4 times by application of high hydrostatic pressure and the discarded balloon flower leaf was used as a substrate. As a result, deglucosylated platycodin D was produced with the highest concentration (3.49 mg/mL) and productivity (581.7 mg/L/h) reported so far. Our results contribute to functional saponin production and the related food industries.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Heliyon Año: 2021 Tipo del documento: Article Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Heliyon Año: 2021 Tipo del documento: Article Pais de publicación: Reino Unido