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The Arabidopsis protein NPF6.2/NRT1.4 is a plasma membrane nitrate transporter and a target of protein kinase CIPK23.
Morales de Los Ríos, Laura; Corratgé-Faillie, Claire; Raddatz, Natalia; Mendoza, Imelda; Lindahl, Marika; de Angeli, Alexis; Lacombe, Benoit; Quintero, Francisco J; Pardo, José M.
Afiliación
  • Morales de Los Ríos L; Institute of Plant Biochemistry and Photosyntheis (IBVF), Consejo Superior de Investigaciones Científicas and Universidad de Sevilla, 41092, Seville, Spain.
  • Corratgé-Faillie C; Biochimie et Physiologie Moléculaire des Plantes, Univ. Montpellier, CNRS, INRAE, 34060, Montpellier Cedex, France.
  • Raddatz N; Institute of Plant Biochemistry and Photosyntheis (IBVF), Consejo Superior de Investigaciones Científicas and Universidad de Sevilla, 41092, Seville, Spain.
  • Mendoza I; Institute of Plant Biochemistry and Photosyntheis (IBVF), Consejo Superior de Investigaciones Científicas and Universidad de Sevilla, 41092, Seville, Spain.
  • Lindahl M; Institute of Plant Biochemistry and Photosyntheis (IBVF), Consejo Superior de Investigaciones Científicas and Universidad de Sevilla, 41092, Seville, Spain.
  • de Angeli A; Biochimie et Physiologie Moléculaire des Plantes, Univ. Montpellier, CNRS, INRAE, 34060, Montpellier Cedex, France.
  • Lacombe B; Biochimie et Physiologie Moléculaire des Plantes, Univ. Montpellier, CNRS, INRAE, 34060, Montpellier Cedex, France.
  • Quintero FJ; Institute of Plant Biochemistry and Photosyntheis (IBVF), Consejo Superior de Investigaciones Científicas and Universidad de Sevilla, 41092, Seville, Spain.
  • Pardo JM; Institute of Plant Biochemistry and Photosyntheis (IBVF), Consejo Superior de Investigaciones Científicas and Universidad de Sevilla, 41092, Seville, Spain. Electronic address: jose.pardo@csic.es.
Plant Physiol Biochem ; 168: 239-251, 2021 Nov.
Article en En | MEDLINE | ID: mdl-34656860
Nitrate and potassium nutrition is tightly coordinated in vascular plants. Physiological and molecular genetics studies have demonstrated that several NPF/NRT1 nitrate transporters have a significant impact on both uptake and the root-shoot partition of these nutrients. However, how these traits are biochemically connected remain controversial since some NPF proteins, e.g. NPF7.3/NRT1.5, have been suggested to mediate K+/H+ exchange instead of nitrate fluxes. Here we show that NPF6.2/NRT1.4, a protein that gates nitrate accumulation at the leaf petiole of Arabidopsis thaliana, also affects the root/shoot distribution of potassium. We demonstrate that NPF6.2/NRT1.4 is a plasma membrane nitrate transporter phosphorylated at threonine-98 by the CIPK23 protein kinase that is a regulatory hub for nitrogen and potassium nutrition. Heterologous expression of NPF6.2/NRT1.4 and NPF7.3/NRT1.5 in yeast mutants with altered potassium uptake and efflux systems showed no evidence of nitrate-dependent potassium transport by these proteins.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arabidopsis / Proteínas de Arabidopsis Idioma: En Revista: Plant Physiol Biochem Asunto de la revista: BIOQUIMICA / BOTANICA Año: 2021 Tipo del documento: Article País de afiliación: España Pais de publicación: Francia
Texto completo
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Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arabidopsis / Proteínas de Arabidopsis Idioma: En Revista: Plant Physiol Biochem Asunto de la revista: BIOQUIMICA / BOTANICA Año: 2021 Tipo del documento: Article País de afiliación: España Pais de publicación: Francia