Your browser doesn't support javascript.
loading
Experimental infection of ostriches with H7N1 low pathogenic and H5N8 clade 2.3.4.4B highly pathogenic influenza A viruses.
Abolnik, Celia; Ostmann, Erich; Woods, Matthew; Wandrag, Daniel B R; Grewar, John; Roberts, Laura; Olivier, Adriaan J.
Afiliación
  • Abolnik C; Department of Production Animal Studies, Faculty of Veterinary Science, University of Pretoria, Old Soutpan Road, Private Bag X04, Onderstepoort, 0110, South Africa. Electronic address: celia.abolnik@up.ac.za.
  • Ostmann E; Department of Production Animal Studies, Faculty of Veterinary Science, University of Pretoria, Old Soutpan Road, Private Bag X04, Onderstepoort, 0110, South Africa.
  • Woods M; Department of Production Animal Studies, Faculty of Veterinary Science, University of Pretoria, Old Soutpan Road, Private Bag X04, Onderstepoort, 0110, South Africa.
  • Wandrag DBR; Department of Production Animal Studies, Faculty of Veterinary Science, University of Pretoria, Old Soutpan Road, Private Bag X04, Onderstepoort, 0110, South Africa.
  • Grewar J; Department of Production Animal Studies, Faculty of Veterinary Science, University of Pretoria, Old Soutpan Road, Private Bag X04, Onderstepoort, 0110, South Africa.
  • Roberts L; Department of Production Animal Studies, Faculty of Veterinary Science, University of Pretoria, Old Soutpan Road, Private Bag X04, Onderstepoort, 0110, South Africa; Western Cape Department of Agriculture, Veterinary Services, Muldersvlei Road, Provate Bag X1, Elsenburg, 7607, South Africa.
  • Olivier AJ; South African Ostrich Business Chamber, Rademeyer Road, Oudtshoorn, 6220, South Africa.
Vet Microbiol ; 263: 109251, 2021 Dec.
Article en En | MEDLINE | ID: mdl-34656859
Infection dynamics data for influenza A virus in a species is important for understanding host-pathogen interactions and developing effective control strategies. Seven-week-old ostriches challenged with H7N1 low pathogenic viruses (LPAIV) or clade 2.3.4.4B H5N8 high pathogenic viruses (HPAIV) were co- housed with non-challenged contacts. Clinical signs, virus shed in the trachea, cloaca, and feather pulp, and antibody responses were quantified over 14 days. H7N1 LPAIV-infected ostriches remained generally healthy with some showing signs of mild conjunctivitis and rhinitis attributed to Mycoplasma co-infection. Mean tracheal virus shedding titres in contact birds peaked 3 days (106.2 EID50 equivalents / ml) and 9 days (105.28 EID50 equivalents / ml) after introduction, lasting for at least 13 days post infection. Cloacal shedding was substantially lower and ceased within 10 days of onset, and low virus levels were detected in wing feather pulp up until day 14. H5N8 HPAIV -infected ostriches showed various degrees of morbidity, with 2/3 mortalities in the in-contact group. Mean tracheal shedding in contact birds peaked 8 days after introduction (106.32 EID50 equivalents/ ml) and lasted beyond 14 days in survivors. Cloacal shedding and virus in feather pulp was generally higher and more consistently positive compared to H7N1 LPAIV, and was also detectable at least until 14 days post infection in survivors. Antibodies against H5N8 HPAIV and H7N1 LPAIV only appeared after day 7 post exposure, with higher titres induced by the HPAIV compared to the LPAIV, and neuraminidase treatment was essential to remove non-specific inhibitors from the H5N8-positive antisera.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Struthioniformes / Subtipo H7N1 del Virus de la Influenza A / Subtipo H5N8 del Virus de la Influenza A / Gripe Aviar Límite: Animals Idioma: En Revista: Vet Microbiol Año: 2021 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Struthioniformes / Subtipo H7N1 del Virus de la Influenza A / Subtipo H5N8 del Virus de la Influenza A / Gripe Aviar Límite: Animals Idioma: En Revista: Vet Microbiol Año: 2021 Tipo del documento: Article Pais de publicación: Países Bajos