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SARS-CoV-2 detection with aptamer-functionalized gold nanoparticles.
Aithal, Srivatsa; Mishriki, Sarah; Gupta, Rohit; Sahu, Rakesh P; Botos, George; Tanvir, Shazia; Hanson, Russell W; Puri, Ishwar K.
Afiliación
  • Aithal S; Department of Mechanical Engineering, McMaster University, Hamilton, Ontario, Canada.
  • Mishriki S; School of Biomedical Engineering, McMaster University, Hamilton, Ontario, Canada.
  • Gupta R; Department of Mechanical Engineering, McMaster University, Hamilton, Ontario, Canada.
  • Sahu RP; Department of Mechanical Engineering, McMaster University, Hamilton, Ontario, Canada; School of Biomedical Engineering, McMaster University, Hamilton, Ontario, Canada; Department of Materials Science and Engineering, McMaster University, Hamilton, Ontario, Canada.
  • Botos G; Genemis Laboratories, Cambridge, Ontario, Canada; Aptavid, New York, USA.
  • Tanvir S; Genemis Laboratories, Cambridge, Ontario, Canada.
  • Hanson RW; Aptavid, New York, USA.
  • Puri IK; Department of Mechanical Engineering, McMaster University, Hamilton, Ontario, Canada; School of Biomedical Engineering, McMaster University, Hamilton, Ontario, Canada; Department of Materials Science and Engineering, McMaster University, Hamilton, Ontario, Canada. Electronic address: ikpuri@mcmaster
Talanta ; 236: 122841, 2022 Jan 01.
Article en En | MEDLINE | ID: mdl-34635231
A rapid detection test for SARS-CoV-2 is urgently required to monitor virus spread and containment. Here, we describe a test that uses nanoprobes, which are gold nanoparticles functionalized with an aptamer specific to the spike membrane protein of SARS-CoV-2. An enzyme-linked immunosorbent assay confirms aptamer binding with the spike protein on gold surfaces. Protein recognition occurs by adding a coagulant, where nanoprobes with no bound protein agglomerate while those with sufficient bound protein do not. Using plasmon absorbance spectra, the nanoprobes detect 16 nM and higher concentrations of spike protein in phosphate-buffered saline. The time-varying light absorbance is examined at 540 nm to determine the critical coagulant concentration required to agglomerates the nanoprobes, which depends on the protein concentration. This approach detects 3540 genome copies/µl of inactivated SARS-CoV-2.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nanopartículas del Metal / COVID-19 Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Talanta Año: 2022 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nanopartículas del Metal / COVID-19 Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Talanta Año: 2022 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Países Bajos