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Customizing biohybrid cryogels to serve as ready-to-use delivery systems of signaling proteins.
Sievers, Jana; Zimmermann, Ralf; Friedrichs, Jens; Pette, Dagmar; Limasale, Yanuar Dwi Putra; Werner, Carsten; Welzel, Petra Birgit.
Afiliación
  • Sievers J; Leibniz-Institut für Polymerforschung Dresden e.V., Max Bergmann Center of Biomaterials Dresden, Hohe Str. 6, 01069, Dresden, Germany.
  • Zimmermann R; Leibniz-Institut für Polymerforschung Dresden e.V., Max Bergmann Center of Biomaterials Dresden, Hohe Str. 6, 01069, Dresden, Germany.
  • Friedrichs J; Leibniz-Institut für Polymerforschung Dresden e.V., Max Bergmann Center of Biomaterials Dresden, Hohe Str. 6, 01069, Dresden, Germany.
  • Pette D; Leibniz-Institut für Polymerforschung Dresden e.V., Max Bergmann Center of Biomaterials Dresden, Hohe Str. 6, 01069, Dresden, Germany.
  • Limasale YDP; Leibniz-Institut für Polymerforschung Dresden e.V., Max Bergmann Center of Biomaterials Dresden, Hohe Str. 6, 01069, Dresden, Germany.
  • Werner C; Leibniz-Institut für Polymerforschung Dresden e.V., Max Bergmann Center of Biomaterials Dresden, Hohe Str. 6, 01069, Dresden, Germany; Technische Universität Dresden, Center for Regenerative Therapies Dresden and Cluster of Excellence Physics of Life, 01062, Dresden, Germany. Electronic address: wer
  • Welzel PB; Leibniz-Institut für Polymerforschung Dresden e.V., Max Bergmann Center of Biomaterials Dresden, Hohe Str. 6, 01069, Dresden, Germany. Electronic address: welzel@ipfdd.de.
Biomaterials ; 278: 121170, 2021 11.
Article en En | MEDLINE | ID: mdl-34628192
Macroporous cryogels have recently gained increasing interest for the controlled administration of signaling proteins in tissue engineering due to an advantageous combination of material properties. However, most of the previously reported cryogel systems did not allow for tunable, sustained protein release. We therefore designed a set of ready-to-use multi-armed polyethylene glycol (starPEG)-heparin cryogel systems containing different amounts of the protein-affine glycosaminoglycan component heparin to enable systematically tunable long-term delivery of different signaling proteins without affecting other cell-instructive properties. Experimental data and mathematical modeling indicate that the macroporous structure causes local differences in the concentration of proteins released into the pores and in the surrounding of the cryogels. As a proof-of-concept for their ready-to-use potential, cryogels pre-functionalized with signaling proteins and cell adhesion-peptides were demonstrated to induce the neuronal differentiation of colonizing pheochromocytoma cells. The elaborated approach opens up new perspectives for cryogels as easily storable and applicable systems for the precision delivery of signaling proteins.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Andamios del Tejido / Criogeles Idioma: En Revista: Biomaterials Año: 2021 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Países Bajos
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Andamios del Tejido / Criogeles Idioma: En Revista: Biomaterials Año: 2021 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Países Bajos