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Identification of miRNAs That Mediate Protective Functions of Anti-Cancer Drugs During White Matter Ischemic Injury.
Baltan, Selva; Sandau, Ursula S; Brunet, Sylvain; Bastian, Chinthasagar; Tripathi, Ajai; Nguyen, Hung; Liu, Helen; Saugstad, Julie A; Zarnegarnia, Yalda; Dutta, Ranjan.
Afiliación
  • Baltan S; Anesthesiology and Perioperative Medicine, 6684Oregon Health and Science University, Portland, OR 97239, USA.
  • Sandau US; 22516Department of Neurosciences, 2569Cleveland Clinic Foundation, Cleveland, OH 44195, USA.
  • Brunet S; Anesthesiology and Perioperative Medicine, 6684Oregon Health and Science University, Portland, OR 97239, USA.
  • Bastian C; 22516Department of Neurosciences, 2569Cleveland Clinic Foundation, Cleveland, OH 44195, USA.
  • Tripathi A; 22516Department of Neurosciences, 2569Cleveland Clinic Foundation, Cleveland, OH 44195, USA.
  • Nguyen H; 22516Department of Neurosciences, 2569Cleveland Clinic Foundation, Cleveland, OH 44195, USA.
  • Liu H; Anesthesiology and Perioperative Medicine, 6684Oregon Health and Science University, Portland, OR 97239, USA.
  • Saugstad JA; Anesthesiology and Perioperative Medicine, 6684Oregon Health and Science University, Portland, OR 97239, USA.
  • Zarnegarnia Y; Anesthesiology and Perioperative Medicine, 6684Oregon Health and Science University, Portland, OR 97239, USA.
  • Dutta R; Anesthesiology and Perioperative Medicine, 6684Oregon Health and Science University, Portland, OR 97239, USA.
ASN Neuro ; 13: 17590914211042220, 2021.
Article en En | MEDLINE | ID: mdl-34619990
We have previously shown that two anti-cancer drugs, CX-4945 and MS-275, protect and preserve white matter (WM) architecture and improve functional recovery in a model of WM ischemic injury. While both compounds promote recovery, CX-4945 is a selective Casein kinase 2 (CK2) inhibitor and MS-275 is a selective Class I histone deacetylase (HDAC) inhibitor. Alterations in microRNAs (miRNAs) mediate some of the protective actions of these drugs. In this study, we aimed to (1) identify miRNAs expressed in mouse optic nerves (MONs); (2) determine which miRNAs are regulated by oxygen glucose deprivation (OGD); and (3) determine the effects of CX-4945 and MS-275 treatment on miRNA expression. RNA isolated from MONs from control and OGD-treated animals with and without CX-4945 or MS-275 treatment were quantified using NanoString nCounter® miRNA expression profiling. Comparative analysis of experimental groups revealed that 12 miRNAs were expressed at high levels in MONs. OGD upregulated five miRNAs (miR-1959, miR-501-3p, miR-146b, miR-201, and miR-335-3p) and downregulated two miRNAs (miR-1937a and miR-1937b) compared to controls. OGD with CX-4945 upregulated miR-1937a and miR-1937b, and downregulated miR-501-3p, miR-200a, miR-1959, and miR-654-3p compared to OGD alone. OGD with MS-275 upregulated miR-2134, miR-2141, miR-2133, miR-34b-5p, miR-153, miR-487b, miR-376b, and downregulated miR-717, miR-190, miR-27a, miR-1959, miR-200a, miR-501-3p, and miR-200c compared to OGD alone. Interestingly, miR-501-3p and miR-1959 were the only miRNAs upregulated by OGD, and downregulated by OGD plus CX-4945 and MS-275. Therefore, we suggest that protective functions of CX-4945 or MS-275 against WM injury maybe mediated, in part, through miRNA expression.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: MicroARNs / Sustancia Blanca / Antineoplásicos Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: ASN Neuro Asunto de la revista: NEUROLOGIA / QUIMICA Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: MicroARNs / Sustancia Blanca / Antineoplásicos Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: ASN Neuro Asunto de la revista: NEUROLOGIA / QUIMICA Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos