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Activation of NLRP3 Inflammasome Complexes by Beta-Tricalcium Phosphate Particles and Stimulation of Immune Cell Migration in vivo.
Maruyama, Kouji; Cheng, Jin-Yan; Ishii, Hidee; Takahashi, Yu; Zangiacomi, Vincent; Satoh, Takatomo; Hosono, Tetsuji; Yamaguchi, Ken.
Afiliación
  • Maruyama K; Experimental Animal Facility, Shizuoka Cancer Center Research Institute, Shizuoka, Japan.
  • Cheng JY; Advanced Analysis Technology Department, Corporate R&D Center, Olympus Corporation, Tokyo, Japan.
  • Ishii H; Experimental Animal Facility, Shizuoka Cancer Center Research Institute, Shizuoka, Japan.
  • Takahashi Y; Experimental Animal Facility, Shizuoka Cancer Center Research Institute, Shizuoka, Japan.
  • Zangiacomi V; Regional Resource Division, Shizuoka Cancer Center Research Institute, Shizuoka, Japan.
  • Satoh T; Advanced Analysis Technology Department, Corporate R&D Center, Olympus Corporation, Tokyo, Japan.
  • Hosono T; Laboratory of Medicinal Microbiology, Yokohama College of Pharmacy, Yokohama, Japan.
  • Yamaguchi K; Shizuoka Cancer Center Hospital and Research Institute, Shizuoka, Japan.
J Innate Immun ; 14(3): 207-217, 2022.
Article en En | MEDLINE | ID: mdl-34619679
Beta-tricalcium phosphate (ß-TCP) serves as a bone substitute in clinical practice because it is resorbable, biocompatible, osteointegrative, and osteoconductive. Particles of ß-TCP are also inflammatory mediators although the mechanism of this function has not been fully elucidated. Regardless, the ability of ß-TCP to stimulate the immune system might be useful for immunomodulation. The present study aimed to determine the effects of ß-TCP particles on NLR family pyrin domain containing 3 (NLRP3) inflammasome complexes. We found that ß-TCP activates NLRP3 inflammasomes, and increases interleukin (IL)-1ß production in primary cultured mouse dendritic cells (DCs) and macrophages, and human THP-1 cells in caspase-1 dependent manner. In THP-1 cells, ß-TCP increased also IL-18 production, and NLRP3 inflammasome activation by ß-TCP depended on phagocytosis, potassium efflux, and reactive oxygen species (ROS) generation. We also investigated the effects of ß-TCP in wild-type and NLRP3-deficient mice in vivo. Immune cell migration around subcutaneously injected ß-TCP particles was reduced in NLRP3-deficient mice. These findings suggest that the effects of ß-TCP particles in vivo are at least partly mediated by NLRP3 inflammasome complexes.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Inflamasomas / Proteína con Dominio Pirina 3 de la Familia NLR Límite: Animals Idioma: En Revista: J Innate Immun Asunto de la revista: ALERGIA E IMUNOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Inflamasomas / Proteína con Dominio Pirina 3 de la Familia NLR Límite: Animals Idioma: En Revista: J Innate Immun Asunto de la revista: ALERGIA E IMUNOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Suiza