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Repurposing the Hybrid Capture 2 (HC2) screening test for whole-genome sequencing of human papillomaviruses.
Debernardi, A; Jarassier, W; Soret, C; Mougin, C; Alizon, S; Bravo, I G; Guenat, D; Prétet, J L; Lepiller, Q.
Afiliación
  • Debernardi A; EA3181, UBFC, LabEx LipSTIC ANR-11-LABX-0021, Besançon, France.
  • Jarassier W; Centre National de Référence Papillomavirus, CHRU de Besançon, Besançon, France.
  • Soret C; EA3181, UBFC, LabEx LipSTIC ANR-11-LABX-0021, Besançon, France.
  • Mougin C; Centre National de Référence Papillomavirus, CHRU de Besançon, Besançon, France.
  • Alizon S; EA3181, UBFC, LabEx LipSTIC ANR-11-LABX-0021, Besançon, France.
  • Bravo IG; Centre National de Référence Papillomavirus, CHRU de Besançon, Besançon, France.
  • Guenat D; French National Center for Scientific Research (CNRS), Laboratory MIVEGEC (CNRS, IRD, Univ Montpellier), Montpellier, France.
  • Prétet JL; French National Center for Scientific Research (CNRS), Laboratory MIVEGEC (CNRS, IRD, Univ Montpellier), Montpellier, France.
  • Lepiller Q; Center for Research on the Ecology and Evolution of Diseases (CREES), Montpellier, France.
Arch Virol ; 166(12): 3421-3425, 2021 Dec.
Article en En | MEDLINE | ID: mdl-34618227
Simple and standardized approaches for genome analysis of human papillomavirus (HPV) by next-generation sequencing are needed. The aim of the study was to develop a protocol for direct deep sequencing of high-risk (hr) HPV strains, based on the widely used commercial Hybrid Capture 2 (QIAGEN) test, without any additional probe design. This protocol was applied to 15 HPV-positive and two HPV-negative cervical samples or cell lines and validated at the genotype level by comparing the sequencing results to those obtained using a commercial genotyping kit. The performance of our protocol, presented in this proof-of-principle study, supports its use for accurate characterization of genetic variants of hrHPV.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias del Cuello Uterino / Infecciones por Papillomavirus / Alphapapillomavirus Tipo de estudio: Diagnostic_studies / Screening_studies Límite: Female / Humans Idioma: En Revista: Arch Virol Año: 2021 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Austria
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias del Cuello Uterino / Infecciones por Papillomavirus / Alphapapillomavirus Tipo de estudio: Diagnostic_studies / Screening_studies Límite: Female / Humans Idioma: En Revista: Arch Virol Año: 2021 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Austria