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Activity-dependent alteration of early myelin ensheathment in a developing sensory circuit.
Chorghay, Zahraa; MacFarquhar, David; Li, Vanessa J; Aufmkolk, Sarah; Schohl, Anne; Wiseman, Paul W; Káradóttir, Ragnhildur Thóra; Ruthazer, Edward S.
Afiliación
  • Chorghay Z; Montreal Neurological Institute-Hospital and Department of Neurology and Neurosurgery, McGill University, Montréal, QC, Canada.
  • MacFarquhar D; Department of Chemistry, Otto Maass Building, McGill University, Montréal, QC, Canada.
  • Li VJ; Department of Physics, Otto Maass Building, McGill University, Montréal, QC, Canada.
  • Aufmkolk S; Montreal Neurological Institute-Hospital and Department of Neurology and Neurosurgery, McGill University, Montréal, QC, Canada.
  • Schohl A; Montreal Neurological Institute-Hospital and Department of Neurology and Neurosurgery, McGill University, Montréal, QC, Canada.
  • Wiseman PW; Department of Chemistry, Otto Maass Building, McGill University, Montréal, QC, Canada.
  • Káradóttir RT; Department of Physics, Otto Maass Building, McGill University, Montréal, QC, Canada.
  • Ruthazer ES; Montreal Neurological Institute-Hospital and Department of Neurology and Neurosurgery, McGill University, Montréal, QC, Canada.
J Comp Neurol ; 530(6): 871-885, 2022 04.
Article en En | MEDLINE | ID: mdl-34599848
Myelination allows for the regulation of conduction velocity, affecting the precise timing of neuronal inputs important for the development and function of brain circuits. In turn, myelination may be altered by changes in experience, neuronal activity, and vesicular release, but the links between sensory experience, corresponding neuronal activity, and resulting alterations in myelination require further investigation. We thus studied the development of myelination in the Xenopus laevis tadpole, a classic model for studies of visual system development and function because it is translucent and visually responsive throughout the formation of its retinotectal system. We begin with a systematic characterization of the timecourse of early myelin ensheathment in the Xenopus retinotectal system using immunohistochemistry of myelin basic protein (MBP) along with third harmonic generation (THG) microscopy, a label-free structural imaging technique. Based on the mid-larval developmental progression of MBP expression in Xenopus, we identified an appropriate developmental window in which to assess the effects of early temporally patterned visual experience on myelin ensheathment. We used calcium imaging of axon terminals in vivo to characterize the responses of retinal ganglion cells over a range of stroboscopic stimulation frequencies. Strobe frequencies that reliably elicited robust versus dampened calcium responses were then presented to animals for 7 d, and differences in the amount of early myelin ensheathment at the optic chiasm were subsequently quantified. This study provides evidence that it is not just the presence but also to the specific temporal properties of sensory stimuli that are important for myelin plasticity.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Retina / Vías Visuales / Techo del Mesencéfalo / Larva / Vaina de Mielina Límite: Animals Idioma: En Revista: J Comp Neurol Año: 2022 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Retina / Vías Visuales / Techo del Mesencéfalo / Larva / Vaina de Mielina Límite: Animals Idioma: En Revista: J Comp Neurol Año: 2022 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos