A magneto-optical biochip for rapid assay based on the Cotton-Mouton effect of γ-Fe2O3@Au core/shell nanoparticles.
J Nanobiotechnology
; 19(1): 301, 2021 Oct 01.
Article
en En
| MEDLINE
| ID: mdl-34598682
BACKGROUND: In the past decades, different diseases and viruses, such as Ebola, MERS and COVID-19, impacted the human society and caused huge cost in different fields. With the increasing threat from the new or unknown diseases, the demand of rapid and sensitive assay method is more and more urgent. RESULTS: In this work, we developed a magneto-optical biochip based on the Cotton-Mouton effect of γ-Fe2O3@Au core/shell magnetic nanoparticles. We performed a proof-of-concept experiment for the detection of the spike glycoprotein S of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The assay was achieved by measuring the magneto-optical Cotton-Mouton effect of the biochip. This magneto-optical biochip can not only be used to detect SARS-CoV-2 but also can be easily modified for other diseases assay. CONCLUSION: The assay process is simple and the whole testing time takes only 50 min including 3 min for the CM rotation measurement. The detection limit of our method for the spike glycoprotein S of SARS-CoV-2 is estimated as low as 0.27 ng/mL (3.4 pM).
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Glicoproteína de la Espiga del Coronavirus
/
Nanopartículas Magnéticas de Óxido de Hierro
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Prueba de COVID-19
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SARS-CoV-2
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COVID-19
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Anticuerpos Antivirales
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
J Nanobiotechnology
Año:
2021
Tipo del documento:
Article
Pais de publicación:
Reino Unido