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Chimeric Helper-Dependent Adenoviruses Transduce Retinal Ganglion Cells and Müller Cells in Human Retinal Explants.
Han, Ian C; Burnight, Erin R; Kaalberg, Emily E; Boyce, Timothy M; Stone, Edwin M; Fingert, John H; Mullins, Robert F; Tucker, Budd A; Wiley, Luke A.
Afiliación
  • Han IC; The University of Iowa Institute for Vision Research, Iowa City, Iowa, USA.
  • Burnight ER; Department of Ophthalmology and Visual Sciences, Carver College of Medicine, The University of Iowa, Iowa City, Iowa, USA.
  • Kaalberg EE; The University of Iowa Institute for Vision Research, Iowa City, Iowa, USA.
  • Boyce TM; Department of Ophthalmology and Visual Sciences, Carver College of Medicine, The University of Iowa, Iowa City, Iowa, USA.
  • Stone EM; The University of Iowa Institute for Vision Research, Iowa City, Iowa, USA.
  • Fingert JH; Department of Ophthalmology and Visual Sciences, Carver College of Medicine, The University of Iowa, Iowa City, Iowa, USA.
  • Mullins RF; The University of Iowa Institute for Vision Research, Iowa City, Iowa, USA.
  • Tucker BA; Department of Ophthalmology and Visual Sciences, Carver College of Medicine, The University of Iowa, Iowa City, Iowa, USA.
  • Wiley LA; The University of Iowa Institute for Vision Research, Iowa City, Iowa, USA.
J Ocul Pharmacol Ther ; 37(10): 575-579, 2021 12.
Article en En | MEDLINE | ID: mdl-34597181
Purpose: Despite numerous recent advances in retinal gene therapy using adeno-associated viruses (AAVs) as delivery vectors, there remains a crucial need to identify viral vectors with the ability to transduce specific retinal cell types and that have a larger carrying capacity than AAV. In this study, we evaluate the retinal tropism of 2 chimeric helper-dependent adenoviruses (HDAds), helper-dependent adenovirus serotype 5 (HDAd5)/3 and HDAd5/35, both ex vivo using human retinal explants and in vivo using rats. Methods: We transduced cultured human retinal explants with HDAd5/3 and HDAd5/35 carrying an eGFP vector and evaluated tropism and transduction efficiency using immunohistochemistry. To assess in vivo transduction efficiency, subretinal injections were performed in wild-type Sprague-Dawley rats. For both explants and subretinal injections, we delivered 10 µL (1 × 106 vector genomes/mL) and assessed tropism at 7- and 14-days post-transduction, respectively. Results: HDAd5/3 and HDAd5/35 both transduced human retinal ganglion cells (RGCs) and Müller cells, but not photoreceptors, in human retinal explants. However, subretinal injections in albino rats resulted in transduction of the retinal pigmented epithelium only, highlighting species-specific differences in retinal tropism and the value of a human explant model when testing vectors for eventual human gene therapy. Conclusions: Chimeric HDAds are promising candidates for the delivery of large genes, multiple genes, or neuroprotective factors to Müller cells and RGCs. These vectors may have utility for targeted therapy of neurodegenerative diseases primarily involving retinal ganglion or Müller cell types, such as glaucoma or macular telangiectasia type 2.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Retina / Terapia Genética / Adenoviridae / Vectores Genéticos Tipo de estudio: Prognostic_studies Límite: Aged / Aged80 / Animals / Humans / Male Idioma: En Revista: J Ocul Pharmacol Ther Asunto de la revista: FARMACOLOGIA / OFTALMOLOGIA / TERAPEUTICA Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Retina / Terapia Genética / Adenoviridae / Vectores Genéticos Tipo de estudio: Prognostic_studies Límite: Aged / Aged80 / Animals / Humans / Male Idioma: En Revista: J Ocul Pharmacol Ther Asunto de la revista: FARMACOLOGIA / OFTALMOLOGIA / TERAPEUTICA Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos