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In vitro antibacterial and anti-inflammatory effects of Anacardium occidentale L. extracts and their toxicity on PBMCs and zebrafish embryos.
Amorim, Luana Souza; Marques Goes, Pedro Everton; Figueiredo, Rebeca Dantas Alves; Souza, Juliana Alves da Costa Ribeiro; Tavares, Josean Fechine; Castellano, Lucio Roberto Cançado; Castro, Ricardo Dias de; Farias, Davi; de Aquino, Sabrina Garcia.
Afiliación
  • Amorim LS; Graduate Program in Dentistry, Center of Health Sciences, Federal University of Paraíba - UFPB, João Pessoa, Brazil.
  • Marques Goes PE; Graduate Program in Dentistry, Center of Health Sciences, Federal University of Paraíba - UFPB, João Pessoa, Brazil.
  • Figueiredo RDA; Graduate Program in Dentistry, Center of Health Sciences, Federal University of Paraíba - UFPB, João Pessoa, Brazil.
  • Souza JADCR; Department of Molecular Biology, Center of Exact and Natural Sciences (CCEN), Federal University of Paraíba - UFPB, João Pessoa, Brazil.
  • Tavares JF; Department of Pharmaceutical Sciences, Center of Health Sciences, Federal University of Paraíba - UFPB, João Pessoa, Brazil.
  • Castellano LRC; Technical School of Health, Center of Health Sciences, Federal University of Paraíba - UFPB, João Pessoa, Brazil.
  • Castro RD; Graduate Program in Dentistry, Center of Health Sciences, Federal University of Paraíba - UFPB, João Pessoa, Brazil.
  • Farias D; Department of Molecular Biology, Center of Exact and Natural Sciences (CCEN), Federal University of Paraíba - UFPB, João Pessoa, Brazil.
  • de Aquino SG; Graduate Program in Dentistry, Center of Health Sciences, Federal University of Paraíba - UFPB, João Pessoa, Brazil.
Drug Chem Toxicol ; 45(6): 2653-2663, 2022 Nov.
Article en En | MEDLINE | ID: mdl-34595971
This study evaluated the in vitro antimicrobial and immunomodulatory action of crude extracts from Anacardium occidentale L. (cashew tree) leaves and bark, and to determine their toxicity to peripheral-blood mononuclear cells (PBMCs) and to zebrafish embryos and larvae. Chemical analysis of extracts was performed by proton nuclear magnetic resonance (1H-NMR). The antibacterial activity was evaluated against selected bacteria strains by determining the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Cytotoxicity of the extracts was assessed using resazurin method, while the effect on production of ROS by PMN leukocytes was measured by luminol. Embryotoxicity to zebrafish was assessed using the fish embryo acute toxicity test (FET) and quantification of toxicity marker enzymes (AChE, LDH, and GST). 1H-NMR results showed anacardic acid as the main component of the extracts. All bacterial species tested were sensitive to the extracts, with MICs ranging from 312.5 to 10,000 µg/mL. Streptococcus mutans and Escherichia coli were the most susceptible species. The extracts promoted cell viability above 75% at concentrations from 1.25 to 80 µg/mL. Both extracts reduced zymosan-induced ROS (p < 0.05) at concentrations of 1, 8, and 80 µg/mL compared to the control. In vivo, there were embryotoxic effects in zebrafish embryos exposed to both extracts through the presence of lethal and sublethal endpoints. The samples also acted by inhibiting the activities of biomarker enzymes. The A. occidentale L. bark and leaf extracts showed antimicrobial potential and modulated ROS production in vitro, but these also showed embryotoxic effects to zebrafish.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Anacardium Límite: Animals Idioma: En Revista: Drug Chem Toxicol Año: 2022 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Anacardium Límite: Animals Idioma: En Revista: Drug Chem Toxicol Año: 2022 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos