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Protective Immunity Induced by TgMIC5 and TgMIC16 DNA Vaccines Against Toxoplasmosis.
Zhu, Yu-Chao; Ma, Li-Juan; Zhang, Ji-Li; Liu, Jian-Fa; He, Yong; Feng, Ji-Ye; Chen, Jia.
Afiliación
  • Zhu YC; Department of Radiology, The Affiliated Hospital of Medical School of Ningbo University, Ningbo University School of Medicine, Ningbo, China.
  • Ma LJ; Department of Integrated Chinese and Western Medicine Oncology, The Affiliated People's Hospital of Ningbo University, Ningbo, China.
  • Zhang JL; Department of Radiology, The Affiliated Hospital of Medical School of Ningbo University, Ningbo University School of Medicine, Ningbo, China.
  • Liu JF; Immunology Innovation Team, Ningbo University School of Medicine, Ningbo, China.
  • He Y; Department of Radiology, The Affiliated Hospital of Medical School of Ningbo University, Ningbo University School of Medicine, Ningbo, China.
  • Feng JY; Department of Hepatobiliary Surgery, The Affiliated People's Hospital of Ningbo University, Ningbo, China.
  • Chen J; Department of Radiology, The Affiliated Hospital of Medical School of Ningbo University, Ningbo University School of Medicine, Ningbo, China.
Front Cell Infect Microbiol ; 11: 686004, 2021.
Article en En | MEDLINE | ID: mdl-34595126
Toxoplasma gondii is an obligate intracellular parasite, which is responsible for a widely distributed zoonosis. Effective vaccines against toxoplasmosis are necessary to protect the public health. The aim of this study is to evaluate the immune efficacy of DNA vaccines encoding TgMIC5 and TgMIC16 genes against T. gondii infection. The recombinant plasmid pVAX-MIC5 and pVAX-MIC16 were constructed and injected intramuscularly in mice. The specific immune responses and protection against challenge with T. gondii RH tachyzoites were evaluated by measuring the cytokine levels, serum antibody concentrations, lymphocyte proliferation, lymphocyte populations, and the survival time. The protection against challenge with the T. gondii RH tchyzoites and PRU cysts was examined by evaluation of the reduction in the brain cyst burden. The results indicated that immunized mice showed significantly increased levels of IgG, IFN-γ, IL-2, IL-12p70, and IL-12p40 and percentages of CD4+ and CD8+ T cells. Additionally, vaccination prolonged the mouse survival time and reduced brain cysts compared with controls. Mouse groups immunized with a two-gene cocktail of pVAX-MIC5 + pVAX-MIC16 were more protected than mouse groups immunized with a single gene of pVAX-MIC5 or pVAX-MIC16. These results demonstrate that TgMIC5 and TgMIC16 induce effective immunity against toxoplasmosis and may serve as a good vaccine candidate against T. gondii infection.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Toxoplasma / Toxoplasmosis / Vacunas Antiprotozoos / Vacunas de ADN Límite: Animals Idioma: En Revista: Front Cell Infect Microbiol Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Toxoplasma / Toxoplasmosis / Vacunas Antiprotozoos / Vacunas de ADN Límite: Animals Idioma: En Revista: Front Cell Infect Microbiol Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza