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Rational design of a highly porous electronic scaffold with concurrent enhancement in cell behaviors and differentiation under electrical stimulation.
Chen, Fang-Jung; Hsiao, Yu-Sheng; Liao, I-Hsiang; Liu, Chun-Ting; Wu, Po-I; Lin, Che-Yu; Cheng, Nai-Chen; Yu, Jiashing.
Afiliación
  • Chen FJ; Department of Chemical Engineering, National Taiwan University, Da'an Dist., Taipei City 10617, Taiwan. jiayu@ntu.edu.tw.
  • Hsiao YS; Department of Materials Science and Engineering, National Taiwan University of Science and Technology, Da'an Dist., Taipei City 10607, Taiwan. yshsiao@mail.ntust.edu.tw.
  • Liao IH; Department of Chemical Engineering, National Taiwan University, Da'an Dist., Taipei City 10617, Taiwan. jiayu@ntu.edu.tw.
  • Liu CT; Department of Chemical Engineering, National Taiwan University, Da'an Dist., Taipei City 10617, Taiwan. jiayu@ntu.edu.tw.
  • Wu PI; Department of Materials Science and Engineering, National Taiwan University of Science and Technology, Da'an Dist., Taipei City 10607, Taiwan. yshsiao@mail.ntust.edu.tw.
  • Lin CY; Institute of Applied Mechanics, National Taiwan University, Da'an Dist., Taipei City 10617, Taiwan.
  • Cheng NC; Department of Surgery, National Taiwan University Hospital, Zhongzheng Dist., Taipei City 10048, Taiwan.
  • Yu J; Department of Chemical Engineering, National Taiwan University, Da'an Dist., Taipei City 10617, Taiwan. jiayu@ntu.edu.tw.
J Mater Chem B ; 9(37): 7674-7685, 2021 09 29.
Article en En | MEDLINE | ID: mdl-34586139
Conductive polymers (CPs) have received increasing attention as promising materials for studying electrophysiological signals in cell and tissue engineering. The combination of CPs with electrical stimulation (ES) could possibly enhance neurogenesis, osteogenesis, and myogenesis. To date, research has been prioritized on capitalizing CPs as two-dimensional (2D) structures for guiding the differentiation. In contrast, relatively little is conducted on the implementation of 3D conductive scaffolds. In this research, we report the synergic assembly of poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) and multi-walled carbon nanotubes (MWCNTs) as a biocompatible, electrically conductive, mechanically robust and structurally porous 3D scaffold. To showcase the bioelectronic utilization, a proof-of-concept demonstration of electrically stimulated cell culture under ES is conducted. The ES effects coupled with the 3D scaffold are promising on pheochromocytoma 12 (PC12), a neuronal cell line, and the ES effect on osteogenesis of human adipose-derived stem cells (hASC) was further studied. PC12 cultured on this PEDOT:PSS/MWCNT 3D scaffolds was induced to differentiate toward a more mature neuronal phenotype with the ES treatment. Furthermore, hASC osteogenesis could be highly promoted in this conductive scaffold with ES. Calcium deposition concentration and osteo-differentiated gene markers were significantly higher with ES. The facile assembly of 3D conductive scaffolds sheds light on both platforms for investigating the 3D microenvironment for electrophysiological simulation of cells and tissues under the ES treatment of in vivo tissue engineering.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Diferenciación Celular / Técnicas de Cultivo de Célula / Estimulación Eléctrica / Electrónica Límite: Animals / Humans Idioma: En Revista: J Mater Chem B Año: 2021 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Diferenciación Celular / Técnicas de Cultivo de Célula / Estimulación Eléctrica / Electrónica Límite: Animals / Humans Idioma: En Revista: J Mater Chem B Año: 2021 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Reino Unido