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Intravenous SPION-labeled adipocyte-derived stem cells targeted to the brain by magnetic attraction in a rat stroke model: An ultrastructural insight into cell fate within the brain.
García-Belda, Paula; Prima-García, Helena; Aliena-Valero, Alicia; Castelló-Ruiz, María; Ulloa-Navas, María José; Ten-Esteve, Amadeo; Martí-Bonmatí, Luis; Salom, Juan B; García-Verdugo, José Manuel; Gil-Perotín, Sara.
Afiliación
  • García-Belda P; Laboratory of Comparative Neurobiology, Institute Cavanilles, University of Valencia, Valencia, Spain.
  • Prima-García H; Instituto de Ciencia Molecular (ICMol), Universitat de València, Paterna, Spain.
  • Aliena-Valero A; Unidad Mixta de Investigación Cerebrovascular, Instituto de Investigación Sanitaria La Fe-Universidad de Valencia, Valencia, Spain.
  • Castelló-Ruiz M; Unidad Mixta de Investigación Cerebrovascular, Instituto de Investigación Sanitaria La Fe-Universidad de Valencia, Valencia, Spain; Departamento de Biología Celular, Biología Funcional y Antropología Física, Universidad de Valencia, Burjassot, Spain.
  • Ulloa-Navas MJ; Laboratory of Comparative Neurobiology, Institute Cavanilles, University of Valencia, Valencia, Spain.
  • Ten-Esteve A; Biomedical Imaging Research Group (GIBI230), La Fe Health Research Institute, Valencia, Spain.
  • Martí-Bonmatí L; Biomedical Imaging Research Group (GIBI230), La Fe Health Research Institute, Valencia, Spain.
  • Salom JB; Unidad Mixta de Investigación Cerebrovascular, Instituto de Investigación Sanitaria La Fe-Universidad de Valencia, Valencia, Spain; Departamento de Fisiología, Universidad de Valencia, Valencia, Spain. Electronic address: salom_jba@gva.es.
  • García-Verdugo JM; Laboratory of Comparative Neurobiology, Institute Cavanilles, University of Valencia, Valencia, Spain. Electronic address: j.manuel.garcia@uv.es.
  • Gil-Perotín S; Laboratory of Central Neuroimmunology, IIS Hospital La Fe, Valencia, Spain. Electronic address: sara.garcia@uv.es.
Nanomedicine ; 39: 102464, 2022 01.
Article en En | MEDLINE | ID: mdl-34583057
Mesenchymal stem cell therapy after stroke is a promising option investigated in animal models and clinical trials. The intravenous route is commonly used in clinical settings guaranteeing an adequate safety profile although low yields of engraftment. In this report, rats subjected to ischemic stroke were injected with adipose-derived stem cells (ADSCs) labeled with superparamagnetic iron oxide nanoparticles (SPIONs) applying an external magnetic field in the skull to retain the cells. Although most published studies demonstrate viability of ADSCs, only a few have used ultrastructural techniques. In our study, the application of a local magnetic force resulted in a tendency for higher yields of SPION-ADSCs targeting the brain. However, grafted cells displayed morphological signs of death, one day after administration, and correlative microscopy showed active microglia and astrocytes associated in the process of scavenging. Thus, we conclude that, although successfully targeted within the brain, SPION-ADSCs viability was rapidly compromised.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Accidente Cerebrovascular / Nanopartículas de Magnetita Límite: Animals Idioma: En Revista: Nanomedicine Asunto de la revista: BIOTECNOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: España Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Accidente Cerebrovascular / Nanopartículas de Magnetita Límite: Animals Idioma: En Revista: Nanomedicine Asunto de la revista: BIOTECNOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: España Pais de publicación: Estados Unidos