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ssc-microRNA-132 targets DACH1 to exert anti-inflammatory and anti-apoptotic effects in Clostridium perfringens beta2 toxin-treated porcine intestinal epithelial cells.
Xie, Kaihui; Yan, Zunqiang; Wang, Wei; Luo, Ruirui; Gao, Xiaoli; Wang, Pengfei; Yang, Qiaoli; Huang, Xiaoyu; Zhang, Juanli; Yang, Jiaojiao; Gun, Shuangbao.
Afiliación
  • Xie K; College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, 730070, China.
  • Yan Z; College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, 730070, China.
  • Wang W; College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, 730070, China.
  • Luo R; College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, 730070, China.
  • Gao X; College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, 730070, China.
  • Wang P; College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, 730070, China.
  • Yang Q; College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, 730070, China.
  • Huang X; College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, 730070, China.
  • Zhang J; College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, 730070, China.
  • Yang J; College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, 730070, China.
  • Gun S; College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, 730070, China; Gansu Research Center for Swine Production Engineering and Technology, Lanzhou, Gansu, 730070, China. Electronic address: gunsbao056@126.com.
Dev Comp Immunol ; 127: 104270, 2022 02.
Article en En | MEDLINE | ID: mdl-34582881
Clostridium perfringens (C. perfringens) type C (CPC) is one of the chief pathogens that causes diarrhea in piglets, and C. perfringens beta2 (CPB2) toxin is the main virulence factor of CPC. Our previous research demonstrated that ssc-microR-132 was differentially expressed in ileal tissues of CPC-mediated diarrheic piglets and healthy piglets, which implied a potential role of ssc-microR-132 in this process. Here, we found that ssc-microR-132 was notably down-regulated in CPB2-exposed intestinal porcine epithelial cells (IPEC-J2), which was consistent with the ileal tissue expression. Moreover, ssc-microR-132 upregulation alleviated CPB2-induced inflammatory damage and apoptosis in IPEC-J2, whereas ssc-microR-132 knockdown presented the opposite effects. Furthermore, the dual-luciferase reporter assay indicated that ssc-microR-132 directly targeted Dachshund homolog 1 (DACH1). Moreover, DACH1 overexpression intensified CPB2-induced inflammatory injury and apoptosis in IPEC-J2. Remarkably, the introduction of DACH1 weakened the anti-inflammatory and anti-apoptotic effects of ssc-microR-132 in CPB2-exposed IPEC-J2. Overall, the results reveal that ssc-microR-132 targeted DACH1 to alleviate CPB2-mediated inflammation and apoptosis in IPEC-J2.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Clostridium perfringens / MicroARNs Límite: Animals Idioma: En Revista: Dev Comp Immunol Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Clostridium perfringens / MicroARNs Límite: Animals Idioma: En Revista: Dev Comp Immunol Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos