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Surface tailored zein as a novel delivery system for hypericin: Application in photodynamic therapy.
Abdelsalam, Ahmed M; Somaida, Ahmed; Ambreen, Ghazala; Ayoub, Abdallah M; Tariq, Imran; Engelhardt, Konrad; Garidel, Patrick; Fawaz, Ibrahim; Amin, Muhammed U; Wojcik, Matthias; Bakowsky, Udo.
Afiliación
  • Abdelsalam AM; Department of Pharmaceutics and Biopharmaceutics, Philipps University of Marburg, Robert-Koch Strasse 4, 35037 Marburg, Germany; Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Al-Azhar University, Assiut, Egypt.
  • Somaida A; Department of Pharmaceutics and Biopharmaceutics, Philipps University of Marburg, Robert-Koch Strasse 4, 35037 Marburg, Germany.
  • Ambreen G; Department of Pharmaceutics and Biopharmaceutics, Philipps University of Marburg, Robert-Koch Strasse 4, 35037 Marburg, Germany.
  • Ayoub AM; Department of Pharmaceutics and Biopharmaceutics, Philipps University of Marburg, Robert-Koch Strasse 4, 35037 Marburg, Germany; Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Zagazig University, Zagazig, Egypt.
  • Tariq I; Department of Pharmaceutics and Biopharmaceutics, Philipps University of Marburg, Robert-Koch Strasse 4, 35037 Marburg, Germany; Punjab University College of Pharmacy, University of Punjab, Allama Iqbal Campus, 54000 Lahore, Pakistan.
  • Engelhardt K; Department of Pharmaceutics and Biopharmaceutics, Philipps University of Marburg, Robert-Koch Strasse 4, 35037 Marburg, Germany.
  • Garidel P; Department of Physical Chemistry, Martin-Luther-University Halle-Wittenberg, Von-Danckelmann-Platz 4, D-06120 Halle/Saale, Germany.
  • Fawaz I; Department of Pharmaceutics and Biopharmaceutics, Philipps University of Marburg, Robert-Koch Strasse 4, 35037 Marburg, Germany.
  • Amin MU; Department of Pharmaceutics and Biopharmaceutics, Philipps University of Marburg, Robert-Koch Strasse 4, 35037 Marburg, Germany.
  • Wojcik M; Department of Pharmaceutics and Biopharmaceutics, Philipps University of Marburg, Robert-Koch Strasse 4, 35037 Marburg, Germany.
  • Bakowsky U; Department of Pharmaceutics and Biopharmaceutics, Philipps University of Marburg, Robert-Koch Strasse 4, 35037 Marburg, Germany. Electronic address: ubakowsky@aol.com.
Mater Sci Eng C Mater Biol Appl ; 129: 112420, 2021 Oct.
Article en En | MEDLINE | ID: mdl-34579929
Zein is an FDA-approved maize protein featured by its manipulative surface and the possibility of fabrication into nanomaterials. Although extensive research has been carried out in zein-based technology, limited work is available for the application of zein in the field of cancer photodynamic therapy (PDT). In this work, we report zein as a carrier for the natural photosensitizer hypericin in the PDT of hepatocellular carcinoma in vitro. Zein was modified through chemical PEGylation to form PEGylated zein micelles that were compared with two zein nanoparticle formulations physically stabilized by either the lecithin/pluronic mixture or sodium caseinate. FT-IR, 1HNMR and HP-SEC MALS approaches were employed to confirm the chemical PEGylation of zein. Our developed zein nanoparticles and micelles were further characterized by photon correlation spectroscopy (PCS) and atomic force microscopy (AFM). The obtained results showed relatively smaller sizes and higher encapsulation of hypericin in the micellar zein than the nanoparticle-based formulations. Phototoxicity on hepatocellular carcinoma (HepG2 cells) manifested a dose-dependent toxicity pattern of all designed zein formulations. However, superior cytotoxicity was prominent for the hypericin-based micelles, which was influenced by the higher cellular uptake profile. Consequently, the treated HepG2 cells manifested a higher level of intracellular generated ROS and disruption of mitochondrial membrane potential, which induced apoptotic cell death. Comparatively, the designed hypericin formulations indicated lower phototoxicity profile in murine fibroblast L929 cells reflecting their safety on normal cells. Our investigations suggested that the surface-modified zein could be employed to enhance the delivery of the hydrophobic hypericin in PDT and pave the way for future in vivo and clinical applications in cancer treatment.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Perileno / Fotoquimioterapia / Zeína / Neoplasias Hepáticas Límite: Animals Idioma: En Revista: Mater Sci Eng C Mater Biol Appl Año: 2021 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Perileno / Fotoquimioterapia / Zeína / Neoplasias Hepáticas Límite: Animals Idioma: En Revista: Mater Sci Eng C Mater Biol Appl Año: 2021 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Países Bajos