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Effects of Elaidic Acid on HDL Cholesterol Uptake Capacity.
Iino, Takuya; Toh, Ryuji; Nagao, Manabu; Shinohara, Masakazu; Harada, Amane; Murakami, Katsuhiro; Irino, Yasuhiro; Nishimori, Makoto; Yoshikawa, Sachiko; Seto, Yutaro; Ishida, Tatsuro; Hirata, Ken-Ichi.
Afiliación
  • Iino T; Division of Cardiovascular Medicine, Graduate School of Medicine, Kobe University, Kobe 650-0017, Japan.
  • Toh R; Central Research Laboratories, Sysmex Corporation, 4-4-4 Takatsukadai, Nishi-ku, Kobe 651-2271, Japan.
  • Nagao M; Division of Evidence-Based Laboratory Medicine, Graduate School of Medicine, Kobe University, Kobe 650-0017, Japan.
  • Shinohara M; Division of Evidence-Based Laboratory Medicine, Graduate School of Medicine, Kobe University, Kobe 650-0017, Japan.
  • Harada A; Division of Epidemiology, Graduate School of Medicine, Kobe University, Kobe 650-0017, Japan.
  • Murakami K; The Integrated Center for Mass Spectrometry, Graduate School of Medicine, Kobe University, Kobe 650-0017, Japan.
  • Irino Y; Central Research Laboratories, Sysmex Corporation, 4-4-4 Takatsukadai, Nishi-ku, Kobe 651-2271, Japan.
  • Nishimori M; Central Research Laboratories, Sysmex Corporation, 4-4-4 Takatsukadai, Nishi-ku, Kobe 651-2271, Japan.
  • Yoshikawa S; Central Research Laboratories, Sysmex Corporation, 4-4-4 Takatsukadai, Nishi-ku, Kobe 651-2271, Japan.
  • Seto Y; Division of Cardiovascular Medicine, Graduate School of Medicine, Kobe University, Kobe 650-0017, Japan.
  • Ishida T; Division of Cardiovascular Medicine, Graduate School of Medicine, Kobe University, Kobe 650-0017, Japan.
  • Hirata KI; Division of Cardiovascular Medicine, Graduate School of Medicine, Kobe University, Kobe 650-0017, Japan.
Nutrients ; 13(9)2021 Sep 04.
Article en En | MEDLINE | ID: mdl-34578988
Recently we established a cell-free assay to evaluate "cholesterol uptake capacity (CUC)" as a novel concept for high-density lipoprotein (HDL) functionality and demonstrated the feasibility of CUC for coronary risk stratification, although its regulatory mechanism remains unclear. HDL fluidity affects cholesterol efflux, and trans fatty acids (TFA) reduce lipid membrane fluidity when incorporated into phospholipids (PL). This study aimed to clarify the effect of TFA in HDL-PL on CUC. Serum was collected from 264 patients after coronary angiography or percutaneous coronary intervention to measure CUC and elaidic acid levels in HDL-PL, and in vitro analysis using reconstituted HDL (rHDL) was used to determine the HDL-PL mechanism affecting CUC. CUC was positively associated with HDL-PL levels but negatively associated with the proportion of elaidic acid in HDL-PL (elaidic acid in HDL-PL/HDL-PL ratio). Increased elaidic acid-phosphatidylcholine (PC) content in rHDL exhibited no change in particle size or CUC compared to rHDL containing oleic acid in PC. Recombinant human lecithin-cholesterol acyltransferase (LCAT) enhanced CUC, and LCAT-dependent enhancement of CUC and LCAT-dependent cholesterol esterification were suppressed in rHDL containing elaidic acid in PC. Therefore, CUC is affected by HDL-PL concentration, HDL-PL acyl group composition, and LCAT-dependent cholesterol esterification. Elaidic acid precipitated an inhibition of cholesterol uptake and maturation of HDL; therefore, modulation of HDL-PL acyl groups could improve CUC.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ácidos Oléicos / Enfermedades Cardiovasculares / HDL-Colesterol Tipo de estudio: Evaluation_studies Límite: Aged / Female / Humans / Male Idioma: En Revista: Nutrients Año: 2021 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ácidos Oléicos / Enfermedades Cardiovasculares / HDL-Colesterol Tipo de estudio: Evaluation_studies Límite: Aged / Female / Humans / Male Idioma: En Revista: Nutrients Año: 2021 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Suiza