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Identification of Abundant and Functional dodecaRNAs (doRNAs) Derived from Ribosomal RNA.
Lambert, Marine; Benmoussa, Abderrahim; Diallo, Idrissa; Ouellet-Boutin, Katheryn; Dorval, Véronique; Majeau, Nathalie; Joly-Beauparlant, Charles; Droit, Arnaud; Bergeron, Alain; Têtu, Bernard; Fradet, Yves; Pouliot, Frédéric; Provost, Patrick.
Afiliación
  • Lambert M; CHU de Québec Research Center/CHUL Pavilion-Université Laval, 2705 boulevard Laurier, Quebec City, QC G1V 4G2, Canada.
  • Benmoussa A; Department of Microbiology, Infectious Diseases and Immunology, Université Laval, Quebec City, QC G1V 4G2, Canada.
  • Diallo I; Faculty of Medicine, Université Laval, Quebec City, QC G1V 0A6, Canada.
  • Ouellet-Boutin K; CHU de Québec Research Center/CHUL Pavilion-Université Laval, 2705 boulevard Laurier, Quebec City, QC G1V 4G2, Canada.
  • Dorval V; Department of Microbiology, Infectious Diseases and Immunology, Université Laval, Quebec City, QC G1V 4G2, Canada.
  • Majeau N; Faculty of Medicine, Université Laval, Quebec City, QC G1V 0A6, Canada.
  • Joly-Beauparlant C; CHU de Québec Research Center/CHUL Pavilion-Université Laval, 2705 boulevard Laurier, Quebec City, QC G1V 4G2, Canada.
  • Droit A; Department of Microbiology, Infectious Diseases and Immunology, Université Laval, Quebec City, QC G1V 4G2, Canada.
  • Bergeron A; Faculty of Medicine, Université Laval, Quebec City, QC G1V 0A6, Canada.
  • Têtu B; CHU de Québec Research Center/CHUL Pavilion-Université Laval, 2705 boulevard Laurier, Quebec City, QC G1V 4G2, Canada.
  • Fradet Y; Department of Microbiology, Infectious Diseases and Immunology, Université Laval, Quebec City, QC G1V 4G2, Canada.
  • Pouliot F; Faculty of Medicine, Université Laval, Quebec City, QC G1V 0A6, Canada.
  • Provost P; CHU de Québec Research Center/CHUL Pavilion-Université Laval, 2705 boulevard Laurier, Quebec City, QC G1V 4G2, Canada.
Int J Mol Sci ; 22(18)2021 Sep 09.
Article en En | MEDLINE | ID: mdl-34575920
Using a modified RNA-sequencing (RNA-seq) approach, we discovered a new family of unusually short RNAs mapping to ribosomal RNA 5.8S, which we named dodecaRNAs (doRNAs), according to the number of core nucleotides (12 nt) their members contain. Using a new quantitative detection method that we developed, we confirmed our RNA-seq data and determined that the minimal core doRNA sequence and its 13-nt variant C-doRNA (doRNA with a 5' Cytosine) are the two most abundant doRNAs, which, together, may outnumber microRNAs. The C-doRNA/doRNA ratio is stable within species but differed between species. doRNA and C-doRNA are mainly cytoplasmic and interact with heterogeneous nuclear ribonucleoproteins (hnRNP) A0, A1 and A2B1, but not Argonaute 2. Reporter gene activity assays suggest that C-doRNA may function as a regulator of Annexin II receptor (AXIIR) expression. doRNAs are differentially expressed in prostate cancer cells/tissues and may control cell migration. These findings suggest that unusually short RNAs may be more abundant and important than previously thought.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ARN Ribosómico / Perfilación de la Expresión Génica / ARN no Traducido / MicroARNs / Transcriptoma Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Int J Mol Sci Año: 2021 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ARN Ribosómico / Perfilación de la Expresión Génica / ARN no Traducido / MicroARNs / Transcriptoma Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Int J Mol Sci Año: 2021 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Suiza