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A Serological Biomarker of Laminin Gamma 1 Chain Degradation Reflects Altered Basement Membrane Remodeling in Crohn's Disease and DSS Colitis.
Lindholm, Majken; Di Sabatino, Antonio; Manon-Jensen, Tina; Mazza, Giuseppe; Madsen, Gunvor I; Giuffrida, Paolo; Pinzani, Massimo; Krag, Aleksander; Karsdal, Morten A; Kjeldsen, Jens; Mortensen, Joachim H.
Afiliación
  • Lindholm M; Biomarkers and Research, Nordic Bioscience, Herlev hovedgade 205-207, 2730, Herlev, Denmark. mlo@nordicbio.com.
  • Di Sabatino A; Department of Medical Gastroenterology, University of Southern Denmark and Odense University Hospital, Odense, Denmark. mlo@nordicbio.com.
  • Manon-Jensen T; First Department of Internal Medicine, San Matteo Hospital Foundation, University of Pavia, Pavia, Italy.
  • Mazza G; Biomarkers and Research, Nordic Bioscience, Herlev hovedgade 205-207, 2730, Herlev, Denmark.
  • Madsen GI; Institute for Liver and Digestive Health, University College of London, London, UK.
  • Giuffrida P; Department of Surgical Pathology, Odense University Hospital, Odense, Denmark.
  • Pinzani M; First Department of Internal Medicine, San Matteo Hospital Foundation, University of Pavia, Pavia, Italy.
  • Krag A; Institute for Liver and Digestive Health, University College of London, London, UK.
  • Karsdal MA; Department of Medical Gastroenterology, University of Southern Denmark and Odense University Hospital, Odense, Denmark.
  • Kjeldsen J; Biomarkers and Research, Nordic Bioscience, Herlev hovedgade 205-207, 2730, Herlev, Denmark.
  • Mortensen JH; Department of Medical Gastroenterology, University of Southern Denmark and Odense University Hospital, Odense, Denmark.
Dig Dis Sci ; 67(8): 3662-3671, 2022 08.
Article en En | MEDLINE | ID: mdl-34561759
BACKGROUND: The laminin gamma 1 chain (LMγ1) is abundant along the crypt-villus axis in the intestinal basement membrane. AIMS: We investigated whether a serological biomarker of laminin degradation was associated with disease activity in patients with Crohn's disease (CD) and in rats with dextran sulfate sodium (DSS)-induced colitis. METHODS: Serum samples from CD patients (n = 43), healthy subjects (n = 19), and Sprague Dawley rats receiving 5-6% DSS water for five days and regular drinking water for 11 days were included in this study. The LG1M biomarker, a neo-epitope degradation fragment of the LMγ1 chain generated by matrix metalloproteinases-9 (MMP-9), was measured in serum to estimate the level of laminin degradation. RESULTS: Serum LG1M was elevated in CD patients with active and inactive disease compared to healthy subjects (p < 0.0001). LG1M distinguished CD patients from healthy subjects, with an area under the curve (AUC) of 0.81 (p < 0.0001). Serum LG1M was decreased in DSS rats compared to controls 2 days after DSS withdrawal, and increased upon reversal of the disease. CONCLUSIONS: Increased serum LG1M in active and inactive CD patients supports the evidence of altered LM expression in both inflamed and non-inflamed tissue. Moreover, lower LG1M levels in the early healing phase of DSS-induced colitis may reflect ongoing mucosal repair.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Membrana Basal / Enfermedad de Crohn / Laminina / Colitis Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Dig Dis Sci Año: 2022 Tipo del documento: Article País de afiliación: Dinamarca Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Membrana Basal / Enfermedad de Crohn / Laminina / Colitis Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Dig Dis Sci Año: 2022 Tipo del documento: Article País de afiliación: Dinamarca Pais de publicación: Estados Unidos