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Common host variation drives malaria parasite fitness in healthy human red cells.
Ebel, Emily R; Kuypers, Frans A; Lin, Carrie; Petrov, Dmitri A; Egan, Elizabeth S.
Afiliación
  • Ebel ER; Department of Biology, Stanford University, Stanford, United States.
  • Kuypers FA; Department of Pediatrics, Stanford University School of Medicine, Stanford, United States.
  • Lin C; Children's Hospital Oakland Research Institute, Oakland, United States.
  • Petrov DA; Department of Pediatrics, Stanford University School of Medicine, Stanford, United States.
  • Egan ES; Department of Biology, Stanford University, Stanford, United States.
Elife ; 102021 09 23.
Article en En | MEDLINE | ID: mdl-34553687
The replication of Plasmodium falciparum parasites within red blood cells (RBCs) causes severe disease in humans, especially in Africa. Deleterious alleles like hemoglobin S are well-known to confer strong resistance to malaria, but the effects of common RBC variation are largely undetermined. Here, we collected fresh blood samples from 121 healthy donors, most with African ancestry, and performed exome sequencing, detailed RBC phenotyping, and parasite fitness assays. Over one-third of healthy donors unknowingly carried alleles for G6PD deficiency or hemoglobinopathies, which were associated with characteristic RBC phenotypes. Among non-carriers alone, variation in RBC hydration, membrane deformability, and volume was strongly associated with P. falciparum growth rate. Common genetic variants in PIEZO1, SPTA1/SPTB, and several P. falciparum invasion receptors were also associated with parasite growth rate. Interestingly, we observed little or negative evidence for divergent selection on non-pathogenic RBC variation between Africans and Europeans. These findings suggest a model in which globally widespread variation in a moderate number of genes and phenotypes modulates P. falciparum fitness in RBCs.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plasmodium falciparum / Malaria Falciparum / Eritrocitos Tipo de estudio: Prognostic_studies Límite: Humans País/Región como asunto: Africa Idioma: En Revista: Elife Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plasmodium falciparum / Malaria Falciparum / Eritrocitos Tipo de estudio: Prognostic_studies Límite: Humans País/Región como asunto: Africa Idioma: En Revista: Elife Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido