High-Throughput BN-PAGE for Mitochondrial Respiratory Complexes.

Vincis Pereira Sanglard, Lilian; Colas des Francs-Small, Catherine

Vincis Pereira Sanglard, Lilian; Colas des Francs-Small, Catherine.

Afiliación

Vincis Pereira Sanglard L; ARC Centre of Excellence for Plant Energy Biology, The University of Western Australia, Crawley, Australia.
Colas des Francs-Small C; ARC Centre of Excellence for Plant Energy Biology, The University of Western Australia, Crawley, Australia. catherine.colasdesfrancs-small@uwa.edu.au.

Methods Mol Biol ; 2363: 111-119, 2022.

Article en En | MEDLINE | ID: mdl-34545490

RESUMEN

Blue native electrophoresis (BN-PAGE) is a highly resolutive method suited to the study of high molecular weight protein complexes between 100 and >3000 kDa. One of the drawbacks of this method is that it is very time-consuming and requires high quantities of purified organelles. Here we describe a high throughput BN-PAGE method allowing to screen libraries of plants potentially altered in respiratory metabolism.

Asunto(s)

Mitocondrias; Electroforesis en Gel Bidimensional; Electroforesis en Gel de Poliacrilamida

Palabras clave

Blue Native PAGE; Immunoblots; Mitochondria; Respiratory complexes

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Mitocondrias Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2022 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Estados Unidos

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