Your browser doesn't support javascript.
loading
Correlative Super-Resolution Optical and Atomic Force Microscopy Reveals Relationships Between Bacterial Cell Wall Architecture and Synthesis in Bacillus subtilis.
Tank, Raveen K G; Lund, Victoria A; Kumar, Sandip; Turner, Robert D; Lafage, Lucia; Pasquina Lemonche, Laia; Bullough, Per A; Cadby, Ashley; Foster, Simon J; Hobbs, Jamie K.
Afiliación
  • Tank RKG; Department of Physics and Astronomy, University of Sheffield, Sheffield S3 7RH, United Kingdom.
  • Lund VA; Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, United Kingdom.
  • Kumar S; The Florey Institute for Host-Pathogen Interactions, University of Sheffield, Sheffield S10 2TN, United Kingdom.
  • Turner RD; Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom.
  • Lafage L; Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, United Kingdom.
  • Pasquina Lemonche L; The Florey Institute for Host-Pathogen Interactions, University of Sheffield, Sheffield S10 2TN, United Kingdom.
  • Bullough PA; Department of Computer Science, University of Sheffield, Sheffield, S1 4DP, United Kingdom.
  • Cadby A; Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, United Kingdom.
  • Foster SJ; The Florey Institute for Host-Pathogen Interactions, University of Sheffield, Sheffield S10 2TN, United Kingdom.
  • Hobbs JK; Department of Physics and Astronomy, University of Sheffield, Sheffield S3 7RH, United Kingdom.
ACS Nano ; 15(10): 16011-16018, 2021 10 26.
Article en En | MEDLINE | ID: mdl-34533301
Understanding how bacteria grow and divide requires insight into both the molecular-level dynamics of ultrastructure and the chemistry of the constituent components. Atomic force microscopy (AFM) can provide near molecular resolution images of biological systems but typically provides limited chemical information. Conversely, while super-resolution optical microscopy allows localization of particular molecules and chemistries, information on the molecular context is difficult to obtain. Here, we combine these approaches into STORMForce (stochastic optical reconstruction with atomic force microscopy) and the complementary SIMForce (structured illumination with atomic force microscopy), to map the synthesis of the bacterial cell wall structural macromolecule, peptidoglycan, during growth and division in the rod-shaped bacterium Bacillus subtilis. Using "clickable" d-amino acid incorporation, we fluorescently label and spatially localize a short and controlled period of peptidoglycan synthesis and correlate this information with high-resolution AFM of the resulting architecture. During division, septal synthesis occurs across its developing surface, suggesting a two-stage process with incorporation at the leading edge and with considerable in-filling behind. During growth, the elongation of the rod occurs through bands of synthesis, spaced by ∼300 nm, and corresponds to denser regions of the internal cell wall as revealed by AFM. Combining super-resolution optics and AFM can provide insights into the synthesis processes that produce the complex architectures of bacterial structural biopolymers.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacillus subtilis / Pared Celular Idioma: En Revista: ACS Nano Año: 2021 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacillus subtilis / Pared Celular Idioma: En Revista: ACS Nano Año: 2021 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Estados Unidos