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Millipore xMap® Luminex (HATMAG-68K): An Accurate and Cost-Effective Method for Evaluating Alzheimer's Biomarkers in Cerebrospinal Fluid.
Hansen, Erika Oliveira; Dias, Natalia Silva; Burgos, Ivonne Carolina Bolaños; Costa, Monica Vieira; Carvalho, Andréa Teixeira; Teixeira, Antonio Lucio; Barbosa, Izabela Guimarães; Santos, Lorena Aline Valu; Rosa, Daniela Valadão Freitas; Ribeiro, Aloisio Joaquim Freitas; Viana, Bernardo Mattos; Bicalho, Maria Aparecida Camargos.
Afiliación
  • Hansen EO; Jenny de Andrade Faria Institute- Reference Center for the Elderly, Hospital das Clínicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
  • Dias NS; Molecular Medicine Program, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
  • Burgos ICB; Neuroscience Program, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
  • Costa MV; Elderly Psychiatry and Psychology Extension Program (PROEPSI), Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
  • Carvalho AT; Adult Health Sciences Applied Program, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
  • Teixeira AL; Molecular Medicine Program, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
  • Barbosa IG; René Rachou Institute, Oswaldo Cruz Foundation (Fiocruz), Belo Horizonte, Brazil.
  • Santos LAV; Department of Psychiatry and Behavioral Sciences, UT Health, Houston, TX, United States.
  • Rosa DVF; Instituto de Ensino e Pesquisa, Santa Casa de Belo Horizonte, Belo Horizonte, Brazil.
  • Ribeiro AJF; Neuroscience Program, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
  • Viana BM; Elderly Psychiatry and Psychology Extension Program (PROEPSI), Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
  • Bicalho MAC; Department of Mental Health, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Front Psychiatry ; 12: 716686, 2021.
Article en En | MEDLINE | ID: mdl-34531769
Background: Alzheimer's disease (AD) biomarkers are of great relevance in clinical research, especially after the AT(N) framework. They enable early diagnosis, disease staging and research with new promising drugs, monitoring therapeutic response. However, the high cost and low availability of the most well-known methods limits their use in low and medium-income countries. In this context, Millipore xMap® Luminex may be a cost-effective alternative. In our study, using INNOTEST® as reference, we assess the diagnostic accuracy of Millipore xMap® and propose a cutoff point for AD. Methods: We performed lumbar puncture of seven older individuals with clinically defined AD, 17 with amnestic mild cognitive impairment (aMCI) and 11 without objective cognitive impairment-control group (CG). Cerebrospinal fluid (CSF) biomarkers concentrations for aB42, p-Tau, and t-Tau were measured by INNOTEST® and Millipore xMap®, and then the techniques were compared to assess the diagnostic accuracy of the new test and to define a cutoff. Results: INNOTEST® and Millipore xMap® measurements showed all correlations >0.8 for the same biomarker, except for t-Tau that was 0.66. Millipore xMap® measurements showed a robust accuracy for all biomarkers, with AUC higher than 0.808 (t-Tau), and the best for Aß42 (AUC = 0.952). The most accurate cutoffs were found at 1012.98 pg/ml (Aß42), 64.54 pg/ml (p-tau), 3251.81 pg/ml (t-tau), 3.370 (t-Tau/Aß42), and 0.059 (p-Tau/Aß42). Conclusion: Given its good accuracy and cost-effectiveness, Milliplex xMap® tests seems a reliable and promising tool, especially for low and middle-income countries.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Health_economic_evaluation / Screening_studies Idioma: En Revista: Front Psychiatry Año: 2021 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Health_economic_evaluation / Screening_studies Idioma: En Revista: Front Psychiatry Año: 2021 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Suiza