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High sodium chloride affects BMP-7 and 1α-hydroxylase levels through NCC and CLC-5 in NRK-52E cells.
Mo, Shu; Cui, Yan; Sun, Kehuan; Wang, Haixia; Peng, Xunqian; Ou, Ling; Lei, Xiaojun; Huang, Mengtian; Mei, Wenhui; Xin, Ling; He, Haibing; Peng, Bojia; Tian, Ya; Wang, Panpan; Li, Xiaoyun; Zhang, Ronghua; Zhu, Xiaofeng.
Afiliación
  • Mo S; The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong 510630, PR China; College of Traditional Chinese Medicine, Jinan University, Guangzhou, Guangdong 510630, PR China; Shenzhen Hospital of Traditional Chinese Medicine, Shenzhen, Guangdong, 518000, PR China.
  • Cui Y; College of Traditional Chinese Medicine, Jinan University, Guangzhou, Guangdong 510630, PR China.
  • Sun K; College of Traditional Chinese Medicine, Jinan University, Guangzhou, Guangdong 510630, PR China.
  • Wang H; College of Traditional Chinese Medicine, Jinan University, Guangzhou, Guangdong 510630, PR China.
  • Peng X; College of Traditional Chinese Medicine, Jinan University, Guangzhou, Guangdong 510630, PR China.
  • Ou L; College of Pharmacy, Jinan University, Guangzhou, Guangdong 510630, PR China.
  • Lei X; College of Clinical Medicine, Jinan University, Guangzhou, Guangdong 510630, PR China.
  • Huang M; College of Clinical Medicine, Jinan University, Guangzhou, Guangdong 510630, PR China.
  • Mei W; College of Traditional Chinese Medicine, Jinan University, Guangzhou, Guangdong 510630, PR China.
  • Xin L; College of Traditional Chinese Medicine, Jinan University, Guangzhou, Guangdong 510630, PR China.
  • He H; College of Pharmacy, Jinan University, Guangzhou, Guangdong 510630, PR China.
  • Peng B; College of Pharmacy, Jinan University, Guangzhou, Guangdong 510630, PR China.
  • Tian Y; College of Pharmacy, Jinan University, Guangzhou, Guangdong 510630, PR China.
  • Wang P; The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong 510630, PR China; Cancer research Institution, Jinan University, Guangzhou, Guangdong, 510630, PR China; Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Informatization, Guangzhou, Guangdong, 510630, PR China
  • Li X; College of Traditional Chinese Medicine, Jinan University, Guangzhou, Guangdong 510630, PR China; Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Informatization, Guangzhou, Guangdong, 510630, PR China.
  • Zhang R; College of Traditional Chinese Medicine, Jinan University, Guangzhou, Guangdong 510630, PR China; College of Pharmacy, Jinan University, Guangzhou, Guangdong 510630, PR China; Cancer research Institution, Jinan University, Guangzhou, Guangdong, 510630, PR China; Guangdong Provincial Key Laboratory o
  • Zhu X; The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong 510630, PR China; Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Informatization, Guangzhou, Guangdong, 510630, PR China. Electronic address: tzhuxf@foxmail.com.
Ecotoxicol Environ Saf ; 225: 112762, 2021 Dec 01.
Article en En | MEDLINE | ID: mdl-34530263
A diet high in sodium chloride (NaCl) can affect renal function damage and increase urinary calcium excretion, leading to bone loss. in renal tubules, Na-Cl co-transporter (NCC) and chloride channel 5 (CLC-5) are involved in regulating urinary calcium excretion. In addition, some cytokines, such as Bone morphogenetic protein 7 (BMP-7) and 1α-hydroxylase, are synthesized by renal tubules, which target on bone and play important roles on bone metabolism. However, the specific mechanisms between NaCl and these ion channels or cytokines still need investigations from many aspects. This study, in culture normal rat renal tubular epithelial NRK-52E cells, showed that high concentrations of NaCl significantly inhibited the cell viability and increased the cell apoptosis. High concentration of NaCl reduce bone mineral density (BMD), as demonstrated by the significantly increased mRNA and protein levels of NCC and osteopontin (OPN), but decreased the levels of CLC-5, BMP-7, and 1α-hydroxylase. In addition, we found that ovariectomized (OVX) rats on a high-salt diet for 12 weeks had altered levels of these indices in the renal cortices. Moreover, the BMD in fourth and fifth lumbar vertebra (LV4 and 5) and femurs were significantly decreased and bone microstructure was destroyed of these rats. We also demonstrated that high concentration of NaCl enhanced the inhibition of these cytokines which is beneficial to increase BMD, induced by modulating ion channels NCC and CLC-5. In conclusion, our results indicate that high concentration of NaCl reduce BMD by regulating ion channels NCC and CLC-5.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cloruro de Sodio / Cloruros Límite: Animals Idioma: En Revista: Ecotoxicol Environ Saf Año: 2021 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
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XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cloruro de Sodio / Cloruros Límite: Animals Idioma: En Revista: Ecotoxicol Environ Saf Año: 2021 Tipo del documento: Article Pais de publicación: Países Bajos