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A Human Neurovascular Unit On-a-Chip.
Lee, Sharon Wei Ling; Rogosic, Renato; Venturi, Claudia; Raimondi, Manuela Teresa; Pavesi, Andrea; Adriani, Giulia.
Afiliación
  • Lee SWL; Singapore Immunology Network (SIgN), Biomedical Sciences Institute, Agency for Science, Technology, and Research (A*STAR), Singapore, Singapore.
  • Rogosic R; Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore (NUS), Singapore, Singapore.
  • Venturi C; Sensor Engineering, Faculty of Science and Engineering, Maastricht University, Maastricht, Netherlands.
  • Raimondi MT; Department of Chemistry, Materials and Chemical Engineering (CMIC) "Giulio Natta", Politecnico di Milano, Milan, Italy.
  • Pavesi A; Department of Chemistry, Materials and Chemical Engineering (CMIC) "Giulio Natta", Politecnico di Milano, Milan, Italy.
  • Adriani G; Institute of Molecular and Cell Biology (IMCB), Agency for Science, Technology, and Research (A*STAR), Singapore, Singapore.
Methods Mol Biol ; 2373: 107-119, 2022.
Article en En | MEDLINE | ID: mdl-34520009
Protection of the central nervous system (CNS) and cerebral homeostasis depend upon the blood-brain barrier (BBB) functions and permeability. BBB restrictive permeability hinders drug delivery for the treatment of several neurodegenerative diseases and brain tumors. Several in vivo animal models and in vitro systems have been developed to understand the BBB complex mechanisms and aid in the design of improved therapeutic strategies. However, there are still many limitations that should be addressed to achieve the structural and chemical environment of a human BBB. We developed a microfluidic-based model of the neurovascular unit. A monolayer of human cerebral endothelial cells (hCMEC-D3) was grown and cocultured with human brain microvascular pericytes (hBMVPC), and human induced pluripotent stem cells differentiated into astrocytes (hiPSC-AC) and neurons (hiPSC-N). To visualize the physiological morphology of each cell type, we used fluorescent cell-specific markers and confocal microscopy. Permeation of fluorescent solutes with different molecular weights was measured to demonstrate that the developed BBB was selectively permeable as a functional barrier.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Dispositivos Laboratorio en un Chip Límite: Animals / Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2022 Tipo del documento: Article País de afiliación: Singapur Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Dispositivos Laboratorio en un Chip Límite: Animals / Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2022 Tipo del documento: Article País de afiliación: Singapur Pais de publicación: Estados Unidos