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Passive sampling, a practical method for wastewater-based surveillance of SARS-CoV-2.
Habtewold, Jemaneh; McCarthy, David; McBean, Edward; Law, Ilya; Goodridge, Larry; Habash, Marc; Murphy, Heather M.
Afiliación
  • Habtewold J; Water, Health and Applied Microbiology Laboratory, Department of Pathobiology, Ontario Veterinary College, University of Guelph, ON, N1G 2W1, Canada.
  • McCarthy D; Environmental and Public Health Microbiology Laboratory (EPHM Lab), Department of Civil Engineering, Monash University, Australia.
  • McBean E; School of Engineering, University of Guelph, ON, N1G 2W1, Canada.
  • Law I; Water, Health and Applied Microbiology Laboratory, Department of Pathobiology, Ontario Veterinary College, University of Guelph, ON, N1G 2W1, Canada.
  • Goodridge L; Department of Food Science, University of Guelph, ON, N1G 2W1, Canada.
  • Habash M; School of Environmental Sciences, University of Guelph, ON, N1G 2W1, Canada.
  • Murphy HM; Water, Health and Applied Microbiology Laboratory, Department of Pathobiology, Ontario Veterinary College, University of Guelph, ON, N1G 2W1, Canada. Electronic address: heather.murphy@uoguelph.ca.
Environ Res ; 204(Pt B): 112058, 2022 03.
Article en En | MEDLINE | ID: mdl-34516976
In search of practical and affordable tools for wastewater-based surveillance of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), three independent field experiments were conducted using three passive sampler sorbents (electronegative membrane, cotton bud, and gauze) in Guelph, Ontario, Canada. Total daily cases during this study ranged from 2 to 17/100,000 people and 43/54 traditionally collected wastewater samples were positive for SARS-CoV-2 with mean detectable concentrations ranging from 8.4 to 1780 copies/ml. Viral levels on the passive samplers were assessed after 4, 8, 24, 48, 72, and 96 hrs of deployment in the wastewater and 43/54 membrane, 42/54 gauze, and 27/54 cotton bud samples were positive. A linear accumulation rate of SARS-CoV-2 on the membranes was observed up to 48 hours, suggesting the passive sampler could adequately reflect wastewater levels for up to two days of deployment. Due the variability in accumulation observed for the cotton buds and gauzes, and the pre-processing steps required for the gauzes, we recommend membrane filters as a simple cost-effective option for wastewater-based surveillance of SARS-CoV-2.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: SARS-CoV-2 / COVID-19 Tipo de estudio: Screening_studies Límite: Humans País/Región como asunto: America do norte Idioma: En Revista: Environ Res Año: 2022 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: SARS-CoV-2 / COVID-19 Tipo de estudio: Screening_studies Límite: Humans País/Región como asunto: America do norte Idioma: En Revista: Environ Res Año: 2022 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Países Bajos