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Trapping and Detection of Single Viruses in an Optofluidic Chip.
Shi, Yuzhi; Nguyen, Kim Truc; Chin, Lip Ket; Li, Zhenyu; Xiao, Limin; Cai, Hong; Yu, Ruozhen; Huang, Wei; Feng, Shilun; Yap, Peng Huat; Liu, Jingquan; Zhang, Yi; Liu, Ai Qun.
Afiliación
  • Shi Y; National Key Laboratory of Science and Technology on Micro/Nano Fabrication, Department of Micro/Nano Electronics, Shanghai Jiao Tong University, Shanghai 200240, China.
  • Nguyen KT; School of Electrical and Electronic Engineering, Nanyang Technological University, 639798 Singapore.
  • Chin LK; School of Electrical and Electronic Engineering, Nanyang Technological University, 639798 Singapore.
  • Li Z; School of Electrical and Electronic Engineering, Nanyang Technological University, 639798 Singapore.
  • Xiao L; School of Electrical and Electronic Engineering, Nanyang Technological University, 639798 Singapore.
  • Cai H; Advanced Fiber Devices and Systems Group, Key Laboratory of Micro and Nano Photonic Structures (MoE), Key Laboratory for Information Science of Electromagnetic Waves (MoE), Shanghai Engineering Research Center of Ultra-Precision Optical Manufacturing, School of Information Science and Technology, Fu
  • Yu R; Institute of Microelectronics, A*STAR (Agency for Science, Technology and Research), 2 Fusionopolis Way, #08-02 Innovis Tower, 138634 Singapore.
  • Huang W; Chinese Research Academy of Environmental Science, 8, Anwai Dayanfang, Beijing 100012, China.
  • Feng S; Key Laboratory of Multifunctional Nanomaterials and Smart Systems, Suzhou Institute of Nano-Tech and Nano-Bionics (SINANO), Chinese Academy of Sciences (CAS), Suzhou 215123, China.
  • Yap PH; School of Electrical and Electronic Engineering, Nanyang Technological University, 639798 Singapore.
  • Liu J; Lee Kong Chian School of Medicine, Nanyang Technological University, 308232 Singapore.
  • Zhang Y; National Key Laboratory of Science and Technology on Micro/Nano Fabrication, Department of Micro/Nano Electronics, Shanghai Jiao Tong University, Shanghai 200240, China.
  • Liu AQ; School of Mechanical and Aerospace Engineering, Nanyang Technological University, 639798 Singapore.
ACS Sens ; 6(9): 3445-3450, 2021 09 24.
Article en En | MEDLINE | ID: mdl-34505501
Accurate single virus detection is critical for disease diagnosis and early prevention, especially in view of current pandemics. Numerous detection methods have been proposed with the single virus sensitivity, including the optical approaches and immunoassays. However, few of them hitherto have the capability of both trapping and detection of single viruses in the microchannel. Here, we report an optofluidic potential well array to trap nanoparticles stably in the flow stream. The nanoparticle is bound with single viruses and fluorescence quantum dots through an immunolabeling protocol. Single viruses can be swiftly captured in the microchannel by optical forces and imaged by a camera. The number of viruses in solution and on each particle can be quantified via image processing. Our method can trap and detect single viruses in the 1 mL serum or water in 2 h, paving an avenue for the advanced, fast, and accurate clinical diagnosis, as well as the study of virus infectivity, mutation, drug inhibition, etc.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Virus / Micromanipulación Tipo de estudio: Diagnostic_studies / Guideline Idioma: En Revista: ACS Sens Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Virus / Micromanipulación Tipo de estudio: Diagnostic_studies / Guideline Idioma: En Revista: ACS Sens Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos