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Clinical significance of serum matrix metalloproteinase 9 and tissue inhibitor of metalloproteinase 1 in the first phase of burn trauma evolution.
Stanciu, Adina Elena; Zamfir-Chiru-Anton, Adina; Stanciu, Marcel Marian; Gherghe, Mirela; Hainarosie, Razvan; Furtunescu, Florentina Ligia; Gheorghe, Dan Cristian.
Afiliación
  • Stanciu AE; Department of Carcinogenesis and Molecular Biology, 'Prof. Dr. Alexandru Trestioreanu' Institute of Oncology, 022328 Bucharest, Romania.
  • Zamfir-Chiru-Anton A; ENT Department, 'Grigore Alexandrescu' Children's Emergency Hospital, 011743 Bucharest, Romania.
  • Stanciu MM; Electrical Engineering Faculty, 'Politehnica' University, 060042, Bucharest, Romania.
  • Gherghe M; Department of Nuclear Medicine, 'Prof. Dr. Alexandru Trestioreanu' Institute of Oncology, 022328 Bucharest, Romania.
  • Hainarosie R; Department of Radiology, 'Carol Davila' University of Medicine and Pharmacy, 050463 Bucharest, Romania.
  • Furtunescu FL; ENT Department, 'Prof. Dr. Dorin Hociota' Institute of Phonoaudiology and Functional ENT and Cervicofacial Surgery, 050751 Bucharest, Romania.
  • Gheorghe DC; ENT Department, 'Carol Davila' University of Medicine and Pharmacy, 050463 Bucharest, Romania.
Exp Ther Med ; 22(4): 1113, 2021 Oct.
Article en En | MEDLINE | ID: mdl-34504567
No prospective study has specifically examined the serum levels of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in the early shock phase of burn-injured patients. Thus, we aimed to detect early changes, activity dynamics, and the predictive value of MMP-9, TIMP-1, and the MMP-9/TIMP-1 ratio to better understand the early repair mechanisms for the development of future therapies for patients with thermal burns. Twenty-five patients with a total body surface area (TBSA) affected by burn <25%, and 30 healthy subjects were enrolled in the study. Serum levels of MMP-9 and TIMP-1 were determined by the ELISA method. Our results showed that MMP-9 concentrations increased immediately after injury and remained on a plateau. In contrast, TIMP-1 showed an upward trend throughout the 7-day study period, and the time course of the MMP-9/TIMP-1 ratio followed the inverse dynamics of TIMP-1. Analysis of the areas under the receiver operating characteristic (ROC) curves (AUC) showed that patients with burn wounds tended to have a MMP-9 value higher than 421.5 ng/ml (AUC=0.979), TIMP-1 value higher than 231.6 ng/ml (AUC=0.908), and MMP-9/TIMP-1 ratio higher than 2.31 (AUC=0.959) (P<0.001). Our findings suggest that although the variations in the two biomarkers were different regarding the time of the initial insult, their ratio is a specific and sensitive indicator of burn evolutivity in patients with a TBSA affected by a burn <25%.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Observational_studies / Prognostic_studies / Risk_factors_studies Idioma: En Revista: Exp Ther Med Año: 2021 Tipo del documento: Article País de afiliación: Rumanía Pais de publicación: Grecia

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Observational_studies / Prognostic_studies / Risk_factors_studies Idioma: En Revista: Exp Ther Med Año: 2021 Tipo del documento: Article País de afiliación: Rumanía Pais de publicación: Grecia