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Immunoelectron Microscopic Characterization of Vasopressin-Producing Neurons in the Hypothalamo-Pituitary Axis of Non-Human Primates by Use of Formaldehyde-Fixed Tissues Stored at -25 °C for Several Years.
Otubo, Akito; Maejima, Sho; Oti, Takumi; Satoh, Keita; Ueda, Yasumasa; Morris, John F; Sakamoto, Tatsuya; Sakamoto, Hirotaka.
Afiliación
  • Otubo A; Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University, Ushimado, Setouchi, Okayama 701-4303, Japan.
  • Maejima S; Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University, Ushimado, Setouchi, Okayama 701-4303, Japan.
  • Oti T; Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University, Ushimado, Setouchi, Okayama 701-4303, Japan.
  • Satoh K; Department of Biological Sciences, Faculty of Science, Kanagawa University, Hiratsuka, Kanagawa 259-1293, Japan.
  • Ueda Y; Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University, Ushimado, Setouchi, Okayama 701-4303, Japan.
  • Morris JF; Department of Anatomy, Kawasaki Medical School, Kurashiki, Okayama 701-0192, Japan.
  • Sakamoto T; Department of Physiology, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan.
  • Sakamoto H; Department of Physiology, Kansai Medical University, Shinmachi, Hirakata, Osaka 573-1191, Japan.
Int J Mol Sci ; 22(17)2021 Aug 25.
Article en En | MEDLINE | ID: mdl-34502087
Translational research often requires the testing of experimental therapies in primates, but research in non-human primates is now stringently controlled by law around the world. Tissues fixed in formaldehyde without glutaraldehyde have been thought to be inappropriate for use in electron microscopic analysis, particularly those of the brain. Here we report the immunoelectron microscopic characterization of arginine vasopressin (AVP)-producing neurons in macaque hypothalamo-pituitary axis tissues fixed by perfusion with 4% formaldehyde and stored at -25 °C for several years (4-6 years). The size difference of dense-cored vesicles between magnocellular and parvocellular AVP neurons was detectable in their cell bodies and perivascular nerve endings located, respectively, in the posterior pituitary and median eminence. Furthermore, glutamate and the vesicular glutamate transporter 2 could be colocalized with AVP in perivascular nerve endings of both the posterior pituitary and the external layer of the median eminence, suggesting that both magnocellular and parvocellular AVP neurons are glutamatergic in primates. Both ultrastructure and immunoreactivity can therefore be sufficiently preserved in macaque brain tissues stored long-term, initially for light microscopy. Taken together, these results suggest that this methodology could be applied to the human post-mortem brain and be very useful in translational research.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Criopreservación / Fijación del Tejido / Sistema Hipotálamo-Hipofisario / Neuronas Límite: Animals Idioma: En Revista: Int J Mol Sci Año: 2021 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Criopreservación / Fijación del Tejido / Sistema Hipotálamo-Hipofisario / Neuronas Límite: Animals Idioma: En Revista: Int J Mol Sci Año: 2021 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Suiza